Matches in SemOpenAlex for { <https://semopenalex.org/work/W2391732009> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2391732009 abstract "To find an easy way to isolate and culture myocardial cells of chickens,the hearts were digested by collagenase typeⅠseveral times and then the supernatant was collected. The digestion was stopped by adding DMEM/F12 supplemented with 20% FBS. Cardiocytes was precipitated by centrifugation,and then added the medium to cardiocytes. Cells were preplated to reduce fibroblast content,and were treated with 5-bromodeoxyuridine (5-Brdu) for 2 days. The identification of cardiocytes was conducted by immunocytochemistry. The results showed that the cells were round before adhesion;12 to 24 hours later,the cells of fusiform and polygon began to adhere and stretch out the parapodium;3 to 4 days later,cell clusters formed;5 to 7 days later,the cells grew together flakily. The identified cells were cardiocytes. Large cardiocytes with well morphology and high livability can be convenient and quickly obtained by digesting of collagenase typeⅠ." @default.
- W2391732009 created "2016-06-24" @default.
- W2391732009 creator A5009966402 @default.
- W2391732009 date "2008-01-01" @default.
- W2391732009 modified "2023-09-27" @default.
- W2391732009 title "Isolation and Primary Culture of Chicken Myocardial Cells" @default.
- W2391732009 hasPublicationYear "2008" @default.
- W2391732009 type Work @default.
- W2391732009 sameAs 2391732009 @default.
- W2391732009 citedByCount "0" @default.
- W2391732009 crossrefType "journal-article" @default.
- W2391732009 hasAuthorship W2391732009A5009966402 @default.
- W2391732009 hasConcept C134018914 @default.
- W2391732009 hasConcept C153911025 @default.
- W2391732009 hasConcept C16685009 @default.
- W2391732009 hasConcept C181199279 @default.
- W2391732009 hasConcept C185592680 @default.
- W2391732009 hasConcept C2775941552 @default.
- W2391732009 hasConcept C2778394429 @default.
- W2391732009 hasConcept C2780381497 @default.
- W2391732009 hasConcept C2781357212 @default.
- W2391732009 hasConcept C35866371 @default.
- W2391732009 hasConcept C43617362 @default.
- W2391732009 hasConcept C54355233 @default.
- W2391732009 hasConcept C55493867 @default.
- W2391732009 hasConcept C71924100 @default.
- W2391732009 hasConcept C7623868 @default.
- W2391732009 hasConcept C81885089 @default.
- W2391732009 hasConcept C86803240 @default.
- W2391732009 hasConcept C87863119 @default.
- W2391732009 hasConcept C89423630 @default.
- W2391732009 hasConceptScore W2391732009C134018914 @default.
- W2391732009 hasConceptScore W2391732009C153911025 @default.
- W2391732009 hasConceptScore W2391732009C16685009 @default.
- W2391732009 hasConceptScore W2391732009C181199279 @default.
- W2391732009 hasConceptScore W2391732009C185592680 @default.
- W2391732009 hasConceptScore W2391732009C2775941552 @default.
- W2391732009 hasConceptScore W2391732009C2778394429 @default.
- W2391732009 hasConceptScore W2391732009C2780381497 @default.
- W2391732009 hasConceptScore W2391732009C2781357212 @default.
- W2391732009 hasConceptScore W2391732009C35866371 @default.
- W2391732009 hasConceptScore W2391732009C43617362 @default.
- W2391732009 hasConceptScore W2391732009C54355233 @default.
- W2391732009 hasConceptScore W2391732009C55493867 @default.
- W2391732009 hasConceptScore W2391732009C71924100 @default.
- W2391732009 hasConceptScore W2391732009C7623868 @default.
- W2391732009 hasConceptScore W2391732009C81885089 @default.
- W2391732009 hasConceptScore W2391732009C86803240 @default.
- W2391732009 hasConceptScore W2391732009C87863119 @default.
- W2391732009 hasConceptScore W2391732009C89423630 @default.
- W2391732009 hasLocation W23917320091 @default.
- W2391732009 hasOpenAccess W2391732009 @default.
- W2391732009 hasPrimaryLocation W23917320091 @default.
- W2391732009 hasRelatedWork W140238201 @default.
- W2391732009 hasRelatedWork W157574943 @default.
- W2391732009 hasRelatedWork W2013519827 @default.
- W2391732009 hasRelatedWork W2025680279 @default.
- W2391732009 hasRelatedWork W2093102541 @default.
- W2391732009 hasRelatedWork W2263950546 @default.
- W2391732009 hasRelatedWork W2363672733 @default.
- W2391732009 hasRelatedWork W2366312071 @default.
- W2391732009 hasRelatedWork W2368814397 @default.
- W2391732009 hasRelatedWork W2410918846 @default.
- W2391732009 hasRelatedWork W2416700840 @default.
- W2391732009 hasRelatedWork W2460411039 @default.
- W2391732009 hasRelatedWork W2463041619 @default.
- W2391732009 hasRelatedWork W2982334237 @default.
- W2391732009 hasRelatedWork W3003107535 @default.
- W2391732009 hasRelatedWork W3030590069 @default.
- W2391732009 hasRelatedWork W3030641407 @default.
- W2391732009 hasRelatedWork W3031216929 @default.
- W2391732009 hasRelatedWork W3032712698 @default.
- W2391732009 hasRelatedWork W976802189 @default.
- W2391732009 isParatext "false" @default.
- W2391732009 isRetracted "false" @default.
- W2391732009 magId "2391732009" @default.
- W2391732009 workType "article" @default.