Matches in SemOpenAlex for { <https://semopenalex.org/work/W2391732885> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2391732885 abstract "The SSR-based molecular markers technique was used to determine the polymorphism change of ovule DNA of Gossypiom hirsutum L.after N~+ ion implantation.The results showed that the ovule DNA polymorphism were changed,which indicated that the DNA sequences of sperm cell were altered.The ovule was developed after the pistils were pollinated by the pollen grains which had been implanted with nitrogen ions.The suppressive subtraction hybridization(SSH) technique was used to isolate the cDNA fragments that showed differential expression between the M_1 progeny developed after the pistils were pollinated by the pollen grains implanted with nitrogen ions and the nature Sumian 22 as control.Two differential expression cDNA libraries were constructed using the cDNAs of the treated M_1 progeny as driver and the cDNAs of untreated M_1 progeny as tester,vice versa.The results demonstrated that the insertion fragments of SSH library were ranging from 450 to 700 bp.50 clones were randomly picked for PCR and sequencing.After matching the results in GenBank dbEST and the Blastn,50 sequences were obtained.Of them,38 of them had 56%～100% similarity with known genes, which was 76% of the total EST;5 sequences had their homology with the sequences in GenBank with unknown function,which was 10% of the total EST;9 were hypothetical protein,which was 18% of the total EST;4 had no similarity with the sequences in GenBank,which might be new genes;or the gene could not search the similarity with the sequences of other species,because the sequences located in the easy-varied 3'-end,which was 8% of the total EST." @default.
- W2391732885 created "2016-06-24" @default.
- W2391732885 creator A5000627937 @default.
- W2391732885 date "2009-01-01" @default.
- W2391732885 modified "2023-09-25" @default.
- W2391732885 title "EFFECTS OF N~+ ION IMPLANTATION INTO POLLEN OF UPLAND COTTON(Gossypium hirsutum L.) ON THE EXPRESSION OF OVULE DNA AND THE cDNA FRAGMENTS OF M_1 PROGENY" @default.
- W2391732885 hasPublicationYear "2009" @default.
- W2391732885 type Work @default.
- W2391732885 sameAs 2391732885 @default.
- W2391732885 citedByCount "0" @default.
- W2391732885 crossrefType "journal-article" @default.
- W2391732885 hasAuthorship W2391732885A5000627937 @default.
- W2391732885 hasConcept C104317684 @default.
- W2391732885 hasConcept C153911025 @default.
- W2391732885 hasConcept C187882448 @default.
- W2391732885 hasConcept C189482833 @default.
- W2391732885 hasConcept C201401135 @default.
- W2391732885 hasConcept C2780618852 @default.
- W2391732885 hasConcept C29172406 @default.
- W2391732885 hasConcept C54355233 @default.
- W2391732885 hasConcept C59822182 @default.
- W2391732885 hasConcept C79029880 @default.
- W2391732885 hasConcept C86803240 @default.
- W2391732885 hasConcept C92324200 @default.
- W2391732885 hasConceptScore W2391732885C104317684 @default.
- W2391732885 hasConceptScore W2391732885C153911025 @default.
- W2391732885 hasConceptScore W2391732885C187882448 @default.
- W2391732885 hasConceptScore W2391732885C189482833 @default.
- W2391732885 hasConceptScore W2391732885C201401135 @default.
- W2391732885 hasConceptScore W2391732885C2780618852 @default.
- W2391732885 hasConceptScore W2391732885C29172406 @default.
- W2391732885 hasConceptScore W2391732885C54355233 @default.
- W2391732885 hasConceptScore W2391732885C59822182 @default.
- W2391732885 hasConceptScore W2391732885C79029880 @default.
- W2391732885 hasConceptScore W2391732885C86803240 @default.
- W2391732885 hasConceptScore W2391732885C92324200 @default.
- W2391732885 hasLocation W23917328851 @default.
- W2391732885 hasOpenAccess W2391732885 @default.
- W2391732885 hasPrimaryLocation W23917328851 @default.
- W2391732885 hasRelatedWork W1974826739 @default.
- W2391732885 hasRelatedWork W1978441412 @default.
- W2391732885 hasRelatedWork W1982326081 @default.
- W2391732885 hasRelatedWork W1989296180 @default.
- W2391732885 hasRelatedWork W2005608701 @default.
- W2391732885 hasRelatedWork W2011187494 @default.
- W2391732885 hasRelatedWork W2067509028 @default.
- W2391732885 hasRelatedWork W2067700101 @default.
- W2391732885 hasRelatedWork W2152886479 @default.
- W2391732885 hasRelatedWork W2156373808 @default.
- W2391732885 hasRelatedWork W2349691871 @default.
- W2391732885 hasRelatedWork W2354157884 @default.
- W2391732885 hasRelatedWork W2370807736 @default.
- W2391732885 hasRelatedWork W2371481956 @default.
- W2391732885 hasRelatedWork W2371598683 @default.
- W2391732885 hasRelatedWork W2375703043 @default.
- W2391732885 hasRelatedWork W2380101541 @default.
- W2391732885 hasRelatedWork W2382696684 @default.
- W2391732885 hasRelatedWork W3140203299 @default.
- W2391732885 hasRelatedWork W3148704138 @default.
- W2391732885 isParatext "false" @default.
- W2391732885 isRetracted "false" @default.
- W2391732885 magId "2391732885" @default.
- W2391732885 workType "article" @default.