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- W2391737558 abstract "Objective To study interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) secretion of human lymphocytes treated with cell-wall deficient Streptercoccus pneumoniae.Methods The L-form of S.pneumoniae was induced by penicillin and the pure cultures of the stable L-forms were isolated.The bacterial form and the stable L-form of S.pneumoniae were heat-inactivated and then mixed with the lymphocytes.The mixture were cultured for 24 hours at 37℃ and then the IL-1β and TNF-α in the cultures were detected with ELISA.Results The results showed that IL-1β and TNF-α were detected in the cultures of the bacterial form/lymphocytes and the L-form/lymphocytes.The concentrations of IL-1β and TNF-α in the cultures of L-forms/lymphocytes were lower than that of parental bacterial/lymphocytes(P0.05).The dependence of secretion on concentration grade was showed in the culture of L-form(r1=0.969,r2=0.960).Conclusion The immunogenicity of L-form of S.pneumoniae could stimulate human lymphocyte to secrete IL-1β and TNF-α,but the immunogenictty is lower than that of baterial form." @default.
- W2391737558 created "2016-06-24" @default.
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- W2391737558 date "2010-01-01" @default.
- W2391737558 modified "2023-09-27" @default.
- W2391737558 title "Cytokine secretion of human lymphocytes stimulated with L-forms of Streptococcus pneumoniae" @default.
- W2391737558 hasPublicationYear "2010" @default.
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