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- W2391760144 abstract "Objective To construct AAV-HSP70 for studying the role and the mechanism of ischemia preconditioning (IP) of kidney.Methods The correct cloning of HSP70 gene in pAAV-IRES-hrGFP was confirmed by restrictive enzyme digestion and sequencing.Eukaryotic expressing vector PAAV-IRES-hrGFP transferred AAV-293 cells with pAAV-Helper,pAAV-RC,then the recombinant AAV-HSP70 was constructed and the high titer of AAV-HSP70 was gained.Results The HSP70 gene was cloned into the adeno-associated virus vector pAAV-IRES-hrGFP,eukaryotic expressing vector PAAV-IRES-hrGFP transferred AAV-293 cells with pAAV-Helper,pAAV-RC and gain the high titer of AAV-HSP70,the titer of the prepared AAV was 2.2×106(pfu/mL) which was enough to transfect in vitro and vivo.Conclusion AAV-HSP70 has been gained successfully,which will provide the base for investigating the role and the mechanism of HSP70 in the ischemia precondition of kidney." @default.
- W2391760144 created "2016-06-24" @default.
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- W2391760144 date "2009-01-01" @default.
- W2391760144 modified "2023-09-24" @default.
- W2391760144 title "Cloning and expression of adeno-associated virus-HSP70" @default.
- W2391760144 hasPublicationYear "2009" @default.
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