Matches in SemOpenAlex for { <https://semopenalex.org/work/W2391763670> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2391763670 abstract "The aim of present study was to clone the CDS sequences of buffalo YY1 gene,and screen its effective shRNA fragments.The cDNA of buffalo fetal fibroblast(BFF) was used as the PCR template,the CDS full-length sequence of buffalo YY1 gene was amplified by using touch down RT-PCR methods.The purified CDS fragment was inserted into pMD18-T vector,and the positive plasmid was identified and sequenced.Fusion expression vector of buffalo YY1 gene was constructed by inserting the YY1 CDS fragment into pEGFP-Nl vector,named as pYY1-EGFPN1.The pYY1-EGFP-N1 plasmid was transfected into BFF cells by Lipofectamine~? LTX reagent.Two shRNA fragments targeting buffalo YY1 gene were designed and synthesized,their lentiviral expression vectors were constructed,named as pSicoR-GFP-YY1 shRNA1/2 respectively.YY1recombinant plasmid(pYY1-EGFP-N1) and shRNA lentiviral expression plasmid(pSicoR-GFPYY1 shRNA1/2) were co-transfected into 293T cells by using Lipo-LTX reagent at the ratio of1:1 or 1:6,pSicoR-GFP and pSicoR-GFP-1864 plasmid were used as blank and negative control respectively.At 72 h after transfection,the cells were harvested,and the total RNA was extracted.The expression of YY1 gene in each transfected cells was detected by qRT-PCR and Westernblot methods.The results showed that the CDS sequence of cloned buffalo YY1 was 1 248 bp,the nucleotide homology of YY1 gene between bovine and buffalo was 99%.The constructed pYY1-EGFP-N1 plasmid could transiently express in BFF cells.The results of qRT-PCR showed that the two designed shRNAs could inhibit the expression of buffalo YY1 mRNA.the shRNA2 fragment had significantly higher inhibition effect than shRNAl(93.64%v 50.77%)(P0.05).The results of Western-blot analysis confirmed that the two shRNAs had inhibition effect on gene expression of buffalo YY1.The above results indicated that the CDS sequence of buffalo YY1 was obtained,two effective shRNA fragments targeting buffalo YY1 gene were selected,which laid foundation for further study the role of YY1 gene in buffalo embryo development." @default.
- W2391763670 created "2016-06-24" @default.
- W2391763670 creator A5040024579 @default.
- W2391763670 date "2014-01-01" @default.
- W2391763670 modified "2023-09-26" @default.
- W2391763670 title "Cloning of Buffalo YYl Gene and Screening Its shRNA Fragments" @default.
- W2391763670 hasPublicationYear "2014" @default.
- W2391763670 type Work @default.
- W2391763670 sameAs 2391763670 @default.
- W2391763670 citedByCount "0" @default.
- W2391763670 crossrefType "journal-article" @default.
- W2391763670 hasAuthorship W2391763670A5040024579 @default.
- W2391763670 hasConcept C104317684 @default.
- W2391763670 hasConcept C142613039 @default.
- W2391763670 hasConcept C153911025 @default.
- W2391763670 hasConcept C187882448 @default.
- W2391763670 hasConcept C190232843 @default.
- W2391763670 hasConcept C22744801 @default.
- W2391763670 hasConcept C33161422 @default.
- W2391763670 hasConcept C40767141 @default.
- W2391763670 hasConcept C49923114 @default.
- W2391763670 hasConcept C54009773 @default.
- W2391763670 hasConcept C54355233 @default.
- W2391763670 hasConcept C67705224 @default.
- W2391763670 hasConcept C86803240 @default.
- W2391763670 hasConcept C92087593 @default.
- W2391763670 hasConceptScore W2391763670C104317684 @default.
- W2391763670 hasConceptScore W2391763670C142613039 @default.
- W2391763670 hasConceptScore W2391763670C153911025 @default.
- W2391763670 hasConceptScore W2391763670C187882448 @default.
- W2391763670 hasConceptScore W2391763670C190232843 @default.
- W2391763670 hasConceptScore W2391763670C22744801 @default.
- W2391763670 hasConceptScore W2391763670C33161422 @default.
- W2391763670 hasConceptScore W2391763670C40767141 @default.
- W2391763670 hasConceptScore W2391763670C49923114 @default.
- W2391763670 hasConceptScore W2391763670C54009773 @default.
- W2391763670 hasConceptScore W2391763670C54355233 @default.
- W2391763670 hasConceptScore W2391763670C67705224 @default.
- W2391763670 hasConceptScore W2391763670C86803240 @default.
- W2391763670 hasConceptScore W2391763670C92087593 @default.
- W2391763670 hasLocation W23917636701 @default.
- W2391763670 hasOpenAccess W2391763670 @default.
- W2391763670 hasPrimaryLocation W23917636701 @default.
- W2391763670 hasRelatedWork W2347707908 @default.
- W2391763670 hasRelatedWork W2348209437 @default.
- W2391763670 hasRelatedWork W2349762164 @default.
- W2391763670 hasRelatedWork W2363683692 @default.
- W2391763670 hasRelatedWork W2365319713 @default.
- W2391763670 hasRelatedWork W2366012763 @default.
- W2391763670 hasRelatedWork W2374364667 @default.
- W2391763670 hasRelatedWork W2375628213 @default.
- W2391763670 hasRelatedWork W2376161078 @default.
- W2391763670 hasRelatedWork W2377242346 @default.
- W2391763670 hasRelatedWork W2377819191 @default.
- W2391763670 hasRelatedWork W2378158835 @default.
- W2391763670 hasRelatedWork W2379370880 @default.
- W2391763670 hasRelatedWork W2380448338 @default.
- W2391763670 hasRelatedWork W2380503735 @default.
- W2391763670 hasRelatedWork W2389360336 @default.
- W2391763670 hasRelatedWork W2407003323 @default.
- W2391763670 hasRelatedWork W2591019456 @default.
- W2391763670 hasRelatedWork W3150034622 @default.
- W2391763670 hasRelatedWork W1839405733 @default.
- W2391763670 isParatext "false" @default.
- W2391763670 isRetracted "false" @default.
- W2391763670 magId "2391763670" @default.
- W2391763670 workType "article" @default.