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- W2391791881 abstract "Porcine reproductive and respiratory syndrome virus(PRRSV) FJ-1 was a newly identified virus isolate in Fujian province.The ORF7 gene of FJ-1 was amplified by RT-PCR and cloned into vector pUCm-T,then subcloned into expression vector of pGEX-4T-3.The recombinant GST-tagged nucleocapsid protein(rN) was expressed in E.coli and the molecular weight was approximately 38 000 as identified by SDS-PAGE and Western blotting.Expression level of the rN protein was approximately 35% of the total bacterial protein and mostly soluble.The rN protein was purified to homogeneity using GST affinity chromatography.Analysis of the rN protein under nonreducing conditions revealed that similar to native protein,the rN protein also possesses homo-dimerization property.An indirect enzyme-linked immunosorbent assay(ELISA) for detecting PRRSV antibody was developed using the purified rN protein as antigen.76 serum samples were detected by the method and the result was compared with that using IDEXX PRRS HerdChek ELISA kit.An identity of 93.4% was revealed between the two ELISA kits and no significant difference(P0.05) was detected.The data indicate the rN protein has the potential usefulness for detection of the PRRSV antibodies." @default.
- W2391791881 created "2016-06-24" @default.
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- W2391791881 date "2007-01-01" @default.
- W2391791881 modified "2023-09-28" @default.
- W2391791881 title "Characterization and application of nucleocapsid protein of porcine reproductive and respiratory syndrome virus expressed in E.coli" @default.
- W2391791881 hasPublicationYear "2007" @default.
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