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- W2391795342 abstract "Referring to the deduced amino acid sequences of β-actin from other vertebrates,two degenerated primers were designed and synthesized for cloning the cDNA of Chinese perch(Siniperca chuatsi) β-actin gene by RT-PCR.The full-length cDNA sequence was further obtained with the method of 3'-and 5'-rapid amplification of cDNA ends(RACE).Genome walker method was applied to get 5'-flanking region of Chinese perch β-actin gene.The complete Chinese perch β-actin cDNA was 1897bp in length,containing an open reading frame of 1128bp(encoding 375 amino acids),flanked by 94bp 5'UTR and 675bp 3'UTR.The sequence analysis reveals that,the identities of β-actin amino acid sequence among Chinese perch,African clawed frog(Xenopus laevis),chicken(Gallus gallus),Norway rat(Rattus norvegicus),house mouse(Mus musculus),and human(Homo sapiens) are more than 96%.This suggests that β-actin is highly conserved.The phylogenetic tree constructed on the basis of β-actin nucleotide sequence describes accurately the relationship among fish,amphibian,bird and mammals.The result supports that β-actin may be investigated further as a good phylogenetic marker.A 1399bp 5'-flanking region of Chinese perch β-actin gene was obtained using genome walker method,a proximal pro-moter element,containing highly conserved CAAT,CArG,and TATA boxes,has been found within the first 200bp up-stream of the start codon ATG.Other potential regulatory elements including GC box,MYOD,YY1,SP1,and GATA also have been found in this 5'-flanking region.The successful cloning of 5'-flanking region of Chinese perch β-actin gene,lays a foundation for future study on transgenic Chinese perch." @default.
- W2391795342 created "2016-06-24" @default.
- W2391795342 creator A5012942230 @default.
- W2391795342 date "2009-01-01" @default.
- W2391795342 modified "2023-09-25" @default.
- W2391795342 title "CLONING AND ANALYSIS OF THE FULL-LENGTH cDNA SEQUENCE AND 5'-FLANKING REGION OF CHINESE PERCH SINIPERCA CHUATSI β-ACTIN GENE" @default.
- W2391795342 hasPublicationYear "2009" @default.
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