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- W2391806602 abstract "Starry flounder Platichthys stellatus is a commercially important marine fish originated in northeast Asia,including China,Japan and Korea.The genetic expression of isozyme of P.stellatus was studied in September 2006.Offspring of wild P.stellatus(44 in num- ber) were collected from the experiment station of Yellow Sea Fisheries Research Institute (YS- FRI) in Yantai.Muscle was collected from each of the samples and stored at-70℃for the test.The muscle samples were homogenized separately in mercaptoethanol buffer (0.05%) at a ratio of 1:3 (g/ml) with ice-bath and centrifuged at 12 000 r/min for 30 rain at 4℃.Vertical polyacrylamide gel electrophoresis (PAGE) was used in examining the muscle samples.Nine isozymes (AMY,EST,GDH.LDH.MDH,ME,POD,SDH and SOD) were examined for their expression in the fish muscle.The isozymes were analyzed by specific isozyme staining meth- ods.Finally,based on the zymograms,the genetic variation of these isozymes was analyzed in terms of their locus number,structure and alleles etc.Altogether,20 loci were scored.Among the scored loci,5 (Amy-3,Gdh,Ldh,Me and Sdh-1) were polymorphic and the mean proportion of polymorphic loci (P) was 25%.The mean effective number of alleles per locus (A_e) was 1.204 6;the mean expected heterozygosity per locus (H_e) was 0.107 8 and the mean observed heterozygosity (H_o) was 0.182 9.In the mean time,the deviation indexes of Hardy-Weinberg equilibrium (d value) of polymorphic locus were tested.All these results indicated that the ge- netic variation of P.stellatus was high,and revealed that the fish had a good potential for farm- ing and genetic breeding." @default.
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- W2391806602 date "2008-01-01" @default.
- W2391806602 modified "2023-09-22" @default.
- W2391806602 title "Isozyme polymorphism in starry flounder Platichthys stellatus" @default.
- W2391806602 hasPublicationYear "2008" @default.
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