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- W2391809011 abstract "Objective To investigate the difference of biological characteristics and growth status between culturing fibrablasts of normal skin and keloid in vitro,the viability of revivificated cells and the change of morpHology after revivificated,to elucidate methods of cells culture in vitro and its cryopereservation,resuscitation and the value of their application. Methods The fibroblasts from eight cases of keloid and eight cases of normal skin were cultured respectively in vitro.Cells were subcultured.Observing cell morpHology of normal skin and keloid fibroblasts, growth curve was used to evaluate the proliferation of cells.Cells in logarithmic growth pHase were placed in refrigerator for gradient cooling and then were preserved in liquid Nitrogen(-196℃) for 2～6 months, the viability of cells was determined by trypan-blue dye,cell morpHology were also observed. Results The results indicated that the fibroblasts isolated from normal skin and keloid exhibited similar morpHology and growth rates,but fibroblasts of keloid were arranged foul-up,overlap,the polarity were disappeared.The primary cells of fibroblasts from keloid grew faster.The frozen cells were resuscitated successfully after 2～6 months,the resuscitated rates were more than 80%,and there were no changed of its morpHology. Conclusion The fibroblasts isolated from normal skin and keloid exhibited similar morpHology and growth rates.In vitro,we established normal skin and keloid fibroblasts cells culturing and its cryopereservation, resuscitation successfully." @default.
- W2391809011 created "2016-06-24" @default.
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- W2391809011 date "2005-01-01" @default.
- W2391809011 modified "2023-09-24" @default.
- W2391809011 title "Study on culture,refrigerated storage,revivification and proliferation of keloid kibroblasts" @default.
- W2391809011 hasPublicationYear "2005" @default.
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