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- W2391919050 abstract "[Objective] The aim of the research was to lay the foundation for obtaining Anthurium andraeanum Lind with tolerance to low temperature. [Method] A. andraeanum was infected by using CBF1 gene mediated by Agrobacterium tumefacien to study the effects of different concn. of acetosyringone (AS) and different infection time on the survival rate of resistant callus and the differentiation rate of resistant buds in A. andraeanum after transformation. [Result] With the recombinant plasmid pBI121-CBF1 as template, PCR amplification was made by using the whole primer sequence of cDNA of CBF1 gene and one fragment with the length of about 650 bp was obtained, which indicated that the recombinanted plasmid containing CBF1 gene was constructe successfully. After screening on the resistant medium for 12 weeks, some calli browned and died. When 100 umol/L AS was used as the inducing materials, the survival rate of resistant calli and the differentiation rate of resistant buds were higher. The infection time of A. tumefacien had certain effects on the survival rate of resistant calli and the differentiation rate of resistant buds after transformation. [Conclusion] When AS concn. was 100 μmol/L and the infection time was 10 min, the survival rate of resistant calli and the differentiation rate of resistant buds were highest, being 48.65% and 11.46% resp." @default.
- W2391919050 created "2016-06-24" @default.
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- W2391919050 date "2008-01-01" @default.
- W2391919050 modified "2023-09-22" @default.
- W2391919050 title "Study on the Transformation of Anthurium andreanum by Agribacterium tumefacien -mediated CBF1 Gene" @default.
- W2391919050 hasPublicationYear "2008" @default.
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