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- W2392012502 abstract "Objective Use quantitative real-time polymerase chain reaction method for detecting the coding gene's expression level of Ag85B,38kDa and MPT64 in Mycobacterium tuberculosis and explore variability of their proteantigen in transcriptional level between the drug-resistant and the sensitive,between the Beijing genotype and non-Beijing genotype and the value of use quantitative real-time polymerase chain reaction diagnosing live Mycobacterium tuberculosis.Methods The specific primers of fbpB,psts1,mpt64 and Siga were designed according to GenBank database.The gene fragments were cloned to pMD18-T simple vector respectively,and recombinant plasmids were purified and quantified spectrophotometrically.We established the method of detecting the expression of fbpB、psts1、mpt64 by using SYBR Green I,and then selected 46 Mycobacterium tuberculosis strains and the Nontuberculosis mycobacteria(2) to do study.qRT-PCR method was used to detecting the gene's expression of fbpB,psts1,mpt64 in 46 Mycobacterium tuberculosis,international standard strain H37Rv and 2 Nontuberculosis mycobacteria.Results The amplification result in live Mycobacterium tuberculosis is S curve,The death and Nontuberculosis mycobacteria can't detecting fluorescencal signal and the curve is linear.The gene's expression of mpt64 in drug-resistant strains is downregulated compare to sensitive strains and H37Rv,The result have statistical significance(t=3.093(P0.01);t=2.545(P0.05)).The gene's expression of psts1 in Beijing genotype is downregulated compare to non-Beijing genotype and H37Rv,The result have statistical significance(t=3.093(P0.01);t=2.545(P0.05)).Conclusion The method of detecting the gene's expression level of fbpB,psts1,mpt64 by real-time PCR have been estabished.This study find the gene's expression of mpt64 in drug-resistant is downregulated compare to sensitive and H37Rv.the gene's expression of psts1 in Beijing genotype is downregulated compare to non-Beijing genotype and H37Rv." @default.
- W2392012502 created "2016-06-24" @default.
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- W2392012502 date "2011-01-01" @default.
- W2392012502 modified "2023-09-27" @default.
- W2392012502 title "The coding gene's expression level of proteantigen in Mycobacterium tuberculosis by qRT-PCR" @default.
- W2392012502 hasPublicationYear "2011" @default.
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