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- W2392103441 abstract "Aim To establish the methods of isolation and culture of neural stem cells and to observe the characteristics of growth proliferation, and induced differentiation of neural stem cells.Methods The neonatal rat subventricular zone tissues were dissociated mechanically and enzymatically. The primary adhesion and passage suspension culture technique were performed to acquire cell clones. Immunocytochemical staining and transmission electron microscope were used to identify the neural stem cells and their differentiated offspring.Results Cell clones could from the neonatal rat subventricular zone tissue after primary and passage culture. There clones were nestin positive and possessed the ability of proliferation and self renewal. The differentiatied cells expressed neurons, astrocytes, oligodendrocytes specific antigen . Conclusion The neural stem cells isolated and cultured by using the present methods have the ability of self renewal and differentiation." @default.
- W2392103441 created "2016-06-24" @default.
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- W2392103441 date "2002-01-01" @default.
- W2392103441 modified "2023-09-22" @default.
- W2392103441 title "Isolation, culture and observation of the neural stem cells from the central nervous system of neonatal rat" @default.
- W2392103441 hasPublicationYear "2002" @default.
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