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- W2392108775 abstract "To identify the characteristics of C17.2 neural stem cells (NSC) and to investigate the toxicity of 6 hydroxydopamine and the protective effect of NURR1 gene. Specific nestin expression in nerve stem cell was detected by immunohistochemistry. Expression of LacZ was detected by X gal staining. Necrosis and apoptosis of neural stem cells induced by 6 hydroxy dopamine at dose of 0.02、0.04、0.06 g/L respectively, with or without the protection of recombinant republic deficit virus with NURR1 gene, were detected by flow cytometry.C17.2 NSC expressed nestin antigen and mostly were stained by X gal. 0.02 g/L 6 hydroxydopamine mainly induced apoptosis of NSC and 0.06g/L dose mostly induced necrosis. The apoptosis and necrosis of C17.2 NSC were decreased by adding NURR1 gene. [Conclusion]Necrosis or apoptosis of NSC induced by 6 hydroxydopamine is closely related to its dose. Overexpression of NURR1 shows protective effect on C17.2 cells." @default.
- W2392108775 created "2016-06-24" @default.
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- W2392108775 date "2004-01-01" @default.
- W2392108775 modified "2023-09-26" @default.
- W2392108775 title "Toxicity of 6 hydroxydopamine to C17.2 Nerve Stem Cells and the Protective Effect of NURR1 Gene" @default.
- W2392108775 hasPublicationYear "2004" @default.
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