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- W2392118067 abstract "Aim To develop and objective,rapid,specific and sensitive UDPE (UDG-Duplex PCR-EIA)assay to detect the pathogen Burkholderia pseudomallei.Method Two sets of primers,targeting FUR(Ferric Uptake Regulator)gene of Burkholderia pseudomallei,were chosen to amplify certain fragments,and an enzyme called UDG(Uracil DNA Glycosylase)was added into the PCR reaction mixture to avoid carry-over caused by PCR products.The PCR products were detected by microwell-hybriadation and Enzyme-Immunoassay(EIA).Different templates were employed to evaluate the specificity and sensitivity of this detection system.Results This system can specifically detect Burkholderia pseudomallei without false positive result in realted species.0.1U of UDG in the PCR reaction mixture can effectively prevent carry-over raised by 109 PCR product molecules.The detection limit of pure DNA template is as low as 10fg/μl;when applied to serial dilution bacteria suspension,the detection limit can be 0.1CFU/μl;When applied to ortificial water Somple,the detection limit can be 10 LFU/μl and the sensitivity of detecting artificial tissue sample and soil sample can be 100CFU/μl.The system developed here can maintain stable at 37℃ fo 7 days.Conclusion The UDPE assay developed in this study can serve as a sensitive and specific method to detect Burkholderia pseudomallei,free from the carry-over of PCR products." @default.
- W2392118067 created "2016-06-24" @default.
- W2392118067 creator A5005111872 @default.
- W2392118067 date "2001-01-01" @default.
- W2392118067 modified "2023-09-27" @default.
- W2392118067 title "AN UDPE ASSAY FOR RAPID AND SENSITIVE DETECTION OF BURKHOLDEIA PSEUDOMALLEI" @default.
- W2392118067 hasPublicationYear "2001" @default.
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