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- W2392131424 abstract "Objective:The aim of this study is to identify the effects of cytokines IL-1β/ IFN-γ on rat skeletal myoblasts. Method:Rat skeletal myoblasts were incubated for 12 h with a combination of IL-1β/ IFN-γ and the differentially expressed genes were determined by a suppression subtractive hybridisation (SSH) method. We randomly selected bacterial colonies from SSH, and chose different length products by PCR, further using sequence analysis. Result:It was found that one skeletal structural protein(tropomyosin) gene was down-regulated, two cDNA sequences were apoptosis-associated genes and another two cDNA were unknown genes. Conclusion:①The results showed that cytokines IL-1β/ IFN-γ down-regulated gene expression of skeletal structural proteins, and up-regulated apoptosis-inducing genes expression, which might causally be involved in the skeletal muscle dysfunction.②Two unknown genes were found, by which the novel clues for the pathogenesis of skeletal intolerance could be established." @default.
- W2392131424 created "2016-06-24" @default.
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- W2392131424 date "2005-01-01" @default.
- W2392131424 modified "2023-09-25" @default.
- W2392131424 title "Effects of cytokines IL-1β/ IFN-γ on rat skeletal myoblasts" @default.
- W2392131424 hasPublicationYear "2005" @default.
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