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- W2392150274 abstract "OBJECTIVE To illustrate the effects of different category of ELSD and different following standard curve on the determination of the component and related impurities of sulfuric gentamycin solution.METHODS The analytical column were phenomex Gemini C18( 250 mm × 4.6 mm,5 μm) and Agilent Zorbax C18( 250 mm × 4.6 mm,5 μm).The column temperature was 30 ℃.The mobile phase consisted of the 0.2 mol·L -1 trifluoroacetic acid solution-methanol ( 94∶6) and ( 96∶4).The flow rate was 0.6 mL· min -1.The drift tube temperature of Waters 2424 was 65 ℃ and the pressure of nebulizing gas was 20 psi.The drift tube temperature of Alltech 2000 was 110 ℃ and the pressure of nebulizing gas was 2.8 L·min -1.The injected volume was 20 μL.RESULTS The different category of ELSD and different following standard curve had no effect on the determination of gentamycin component,but had influence on related impurities of sulfuric gentamycin solution.CONCLUSION The Norm of peak area could be used to determine the gentamycin component instead of the following standard curve and the operation and calculation was more simplified.The related impurities should be controlled to ensure the quality of sulfuric gentamycin solution,and the following standard curve should be used." @default.
- W2392150274 created "2016-06-24" @default.
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- W2392150274 date "2010-01-01" @default.
- W2392150274 modified "2023-09-23" @default.
- W2392150274 title "Analysis of Gentamycin component and related impurities by HPLC-ELSD" @default.
- W2392150274 hasPublicationYear "2010" @default.
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