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- W2392156939 abstract "OBJECTIVE To investigate the impact of different extraction methods on the protein content and gel electrophoresis characteristic bands of the Centipede in the prescription of QianYu,and taking it as a basis for the research of post-extraction crafts of QianYu.METHODS The total proteins in the extract were determined using UV-Vis spectrophotometer;and its gel electrophoresis bands were acquired using polyacrylamide gel electrophoresis;the relation of protein content at different time and different extraction methods of Centipede in the prescription of QianYu was investigated by t test.RESULTS The protein content of Centipede extracted with 30 min was higher than 15min and 45min using decoction method and ultrasound method;and the protein contents of ultrasound method at each time point were about 2 times higher than decoction method;The protein content of decoction test solution of QianYu compound was higher than the decocted-later time 15 min and 30 min solution.The molecular weights of Centipede were in the range 10 kDa-100 kDa,at about 16 kDa.The gray value of protein gel electrophoresis characteristic bands dealed with ultrasound method was significantly higher than the decoction method at the corresponding time.The gray value of protein gel electrophoresis of decoction test solution was the largest in the prescription of QianYu.CONCLUSION Extraction method greatly affected the total proteins of animal drugs of centipede and gel electrophoresis bands in the prescription of QianYu,suggesting that we should pay attention to the research of post-preparation crafts." @default.
- W2392156939 created "2016-06-24" @default.
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- W2392156939 date "2012-01-01" @default.
- W2392156939 modified "2023-09-26" @default.
- W2392156939 title "Effects of different extraction methods on content and gel electrophoresis characteristic spectrum of Centipede total protein from Qianyu" @default.
- W2392156939 hasPublicationYear "2012" @default.
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