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- W2392171501 abstract "Objective To know the effects of di-2-ethylhexyl phthalate(DEHP) on the levels of progesterone in blood of rats and the expression of steroidogenic acute regulatory protein(StAR) mRNA and cytochrome P450 cholesterol side chain cleavage enzyme(P450scc) mRNA in ovarian tissue of rats. Methods Thirty-two female SD rats were randomly divided into control group and three experimental groups,eight in each, and treated with corn oil for control and DEHP for experimental groups at the doses of 10,100,1 000 mg/kg body weight through gavage,the treatments were conducted every day for 10 consecutive days. The blood was collected and progesterone level in serum was measured by electro-chemiluminescence immunoassay. The ovarian tissue of rats was collected to extract total mRNA and RT-PCR was used to analyze the levels of StAR and P450 scc mRNA. Results The level of progesterone in 1 000 mg/kg body weight group was higher than those in other groups. Compared with the control group,the expression levels of StAR mRNA in 100 mg/kg body weight and 1 000 mg/kg body weight groups were significantly lower(P0.05, P0.01). Conclusion DEHP exposure may increase the level of progesterone synthesis in rats by decreasing the transcription levels of StAR and P450 scc mRNA." @default.
- W2392171501 created "2016-06-24" @default.
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- W2392171501 date "2014-01-01" @default.
- W2392171501 modified "2023-09-24" @default.
- W2392171501 title "Effects of di-2-ethylhexyl phthalate exposure on progesterone synthesis of ovary and expression of related genes in pubertal female rats" @default.
- W2392171501 hasPublicationYear "2014" @default.
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