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- W2392173820 abstract "The P32 gene of capripoxvirus was multiplied by PCR and cloned into the pMD18T vector.After sequencing the gene,the P32 gene was separately cloned into the Bac-to-Bac~(○R) Baculovirus Expression System transfer vector pFastBac1 and pFastBacHTa,then the recombinant vectors were transfered into E.coli DH10Bac,which contains baculovirus shuttle vector Bacmid.We got two recombinant bacmids:Bac-Bac1-P32 and Bac-BacHT-P32.The two recombinant bacmids were approved to contain P32 gene by PCR test,so they shall be used to transfect Sf-9 insect cells,and then we shall separately get single recombinant P32 protein and recombinant 6-polyhistidine-P32 protein." @default.
- W2392173820 created "2016-06-24" @default.
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- W2392173820 date "2004-01-01" @default.
- W2392173820 modified "2023-09-24" @default.
- W2392173820 title "Cloning of the gene of capripoxvirus major antigen P32 and construction of its baculovirus expression vector" @default.
- W2392173820 hasPublicationYear "2004" @default.
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