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• W2392190282 abstract "A feeding experiment was conducted to investigate the effects of Bacillus and traditional Chinese medicines(TCM) on growth and intestinal bacterial flora of shrimp L.vannamei.Four experimental diets were formulated by adding the Bacillus and TCM to basil diet(C): 0.2% TCM(M),0.20% TCM+0.30% Bacillus(BM1),0.10% TCM+0.15% Bacillus(BM2),0.30% Bacillus(B).The concentration of Bacillus was 109 CFU·g-1.The TCM consisted of a mixture of herbs and plant materials Isatis tinctoria L.,Isatis indigodica Fort,Forsythia suspersa Vahl,Corydalis bungeana Turez,Pogostemon cablin(blanco) Benth and Astragalus menbranaceus(Fisch.) Bge.Acclimatized shrimp with an average initial weight of 1.91±0.03 g were selected and stocked into 15 cement tanks at a stocking density of 80 shrimp.Each aquarium contained 1500L of sand filtered seawater with a flow rate of 0.9L·min-1.The water was continuously aerated with two air stones.Each diet was given to shrimp four times(07:00,12:00,17:00,23:00) a day to saturation for a period of 56 days.During the experiment period,the water temperature fluctuated between 19.6 and 25.2oC,salinity between 30 and 31 psu.At the end of feeding trial,survival ranged from 95.83% to 98.33% with no significant difference(P0.05) among all groups.Growth measured as final weight was slightly higher(P0.05) in shrimp fed with BM1,M and B than that with C,and there was a significant difference(P0.05) between BM2 and C.In case of weight gain(WG) and specific growth rate(SGR),the shrimp fed with M,BM1 and BM2 exhibited significantly(P0.05) higher values than that with C and B.Of the five diets,BM2 exhibited the best performance,followed by BM1.The combination of Bacillus spp.and TCM,therefore,proved to be more effective and economically viable.The total bacteria counts and Vibrio counts in shrimp fed with M were the most;the counts with shrimp fed with BM1 and BM2 were the least.In each group 30 bacterial strains were purified and identified.These bacterial strains belonged to 12,8,6,5,7 genera in C,M,BM1,BM2 and B,respectively.Most of the bacteria were gram negative bacteria;they counted a per-centage of 86% to 93% in each group.Vibrio spp.,Photobacterium spp.and Pseudomonas spp.were the predominant bacterial flora;however,their values varied in different groups.The results showed that the diets supplemented with TCM and Bacillus also changed the number and composition of intestinal bacterial flora." @default.
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• W2392190282 title "Effects of dietary Bacillus spp. and traditional Chinese medicines on growth and intestinal bacterial flora of shrimp Litopenaeus vannamei" @default.
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