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- W2392203742 abstract "Plant Mlo mutants had broad-spectrum disease resistance to powdery mildew.Using 24 wheat varieties with different powdery mildew disease resistance as experimental material,and primers designed from conserved domain of Mlo protein,resistance gene analogs(RGA) method was conducted to explore a rapid and efficient way for screening and using powdery mildew resistance gene.The results showed that sequence variation in genomic DNA of resistance material to the homologous region of degenerate primers was more than susceptible material,which caused low amplification efficiency.The amplification result of degenerate primers was similar in resistant and susceptible materials,so the relationship between wheat powdery mildew gene and amplified RGA could not be judged only from this result.Resistance-related RGA needed further screening through downstream transformation,cloning and sequencing." @default.
- W2392203742 created "2016-06-24" @default.
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- W2392203742 date "2012-01-01" @default.
- W2392203742 modified "2023-09-22" @default.
- W2392203742 title "Amplification of Powdery Mildew Resistance Gene in Wheat Using Conserved Domain MJ4-MrcD2 of Mlo Protein" @default.
- W2392203742 hasPublicationYear "2012" @default.
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