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- W2392216403 abstract "Objective:To acquire high quality HSP90α protein.Methods:Hsp90α cDNA 5' end 424 bp fragment was amplified by PCR.Three bases were mutated,so NcoI restriction enzyme site was produced.The PCR product was successively inserted into intermediate vector,by some restriction enzyme hsp90α 3' end fragment was inserted into the vector.Thus,a clone was obtained which contains whole fragment of hsp90α cDNA with NcoI restriction enzyme site.After cleaved with NcoI and BamHI,the hsp90α cDNA was cloned into pET 16 blxpression vector.Results:tSDS PAGE gel electrophoresis showed that a protein band with molecular weight of 83 kD appeared as the expected size after transformation and inductionin the host bacteria (DE3).Western blot analysis showed that the expressed protein was hybridized with the antibody to HSP90α. Conclusion:HSP90α expressing plasmid was constructed." @default.
- W2392216403 created "2016-06-24" @default.
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- W2392216403 date "2000-01-01" @default.
- W2392216403 modified "2023-09-25" @default.
- W2392216403 title "Expression of HSP90α in E. coli" @default.
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