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- W2392233710 abstract "Objective To generate and identify an AAV-VEGF165vector using a hypoxic response element promoter to control the expression of hVEGF165.Methods AVV vectors were prepared by using the three-plasmid cotransfection system.The AAV vector was cotransfected with two helper plasmids into HEK293T cells by calcium phosphate method,where one helper(pAAV-RC) mediates AAV vector replication,the other(pHelper) has AAV rep and cap genes.Myocardiocytes were isolated from neonatal mouse hearts and infected with AAV-HRE9-VEGF165 vector.The expression of hVEGF165 protein was shown with immunofluorescence.Results The sequence result of plasmid AAV-HRE9-VEGF165 was identical to that of human VEGF165 published by NCBI in GenBanK.The expression of hVEGF165 in myocardiocytes was elicited under anoxic condition,but not under normal non-anoxic condition.Conclusion An adeno-associated virus vector(rAAV) was generated,containing a HRE(hypoxic response element promotor) and the objective gene VEGF165.The rAAV could infect myocardiocytes to transfer objective gene,and HRE9 could timely regulate the objective gene in response to the condition of myocardial ischemia." @default.
- W2392233710 created "2016-06-24" @default.
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- W2392233710 date "2007-01-01" @default.
- W2392233710 modified "2023-09-26" @default.
- W2392233710 title "The generation and verification of an adeno-associated virus vector using a hypoxic response element promoter to control the expression of human-VEGF_(165)" @default.
- W2392233710 hasPublicationYear "2007" @default.
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