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- W2392242597 abstract "In order to identify genes induced during the drought stress response in flowering maize(Zea mays),suppression subtractive hybridization(SSH) was performed using cDNAs prepared from flowering maize leaves treated with drought stress for one day and 7days as testers and cDNAs from unstressed flowering maize leaves as drivers.Two subtractive cDNA libraries were constructed,in which the rate of recombination was 95% and the size of inserts ranged from 300?bp to 600?bp.Analysis of sequences from the positive clones picked randomly revealed that many drought stress associated genes,including dehydrin,sucrose synthase 3,betaine aldehyde dehydrogenase and DRE binding factor 1,etc.,were obtained.The successful construction of the two subtracted cDNA libraries enables us to identify new different expressed genes involved in the resistance mechanism of flowering maize." @default.
- W2392242597 created "2016-06-24" @default.
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- W2392242597 date "2006-01-01" @default.
- W2392242597 modified "2023-09-25" @default.
- W2392242597 title "Construction and analysis of suppression subtractive hybridization library from the leaves of maize flowering plants under Drought Stress" @default.
- W2392242597 hasPublicationYear "2006" @default.
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