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- W2392281085 abstract "Objective To investigate staining methods for two-dimensional gel(2-DE)electrophoresis in multidrug resistance of gastric cancer.Methods Cultured vincristine-resistant human gastric cancer cell line SGC7901/VCR and its parental cell line SGC7901.Variant amount protein of those cells were separated by 2-DE.Gels were stained with silver nitrate or colloidal Coomassie brilliant blue,and scanned by Image Scanner.Results Well-resolved,reproducible 2-DE patterns of SGC7901/VCR and SGC7901 were established.Silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.Gels stained with colloidal Coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.Conclusion Two staining methods were influenced largely by the sum of protein samples,properly selection may be helpful for further study with proteomics in multidrug resistance of gastric cancer." @default.
- W2392281085 created "2016-06-24" @default.
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- W2392281085 date "2008-01-01" @default.
- W2392281085 modified "2023-09-24" @default.
- W2392281085 title "Analysis for staining methods of two-dimentional patterns in drug resistant gastric carcinoma cells research" @default.
- W2392281085 hasPublicationYear "2008" @default.
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