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- W2392282229 abstract "AIM:To construct the eukaryotic expression vector that specifically drives pro-caspase-7 by prostate-specific antigen promoter. By employing the prostate-specific antigen promoter (PSAP), we investigated whether PSAP driven pro caspase 7 could induce death of prostate cancer cells and whether the cytotoxicity was restricted to cells of prostate origin. METHODS: We extracted genomic DNA from benign prostate tissues and obtained prostate specific antigen promoter(PSAP)fragment by polymerase chain reaction (PCR).Using gene technology, we replaced the pCMV on pCDNA3 by PSAP and obtained PSAP pCDNA3 vector. Then the pCMV on pIRES2 EGFP and pIRES2 EGFP Csp7 was replaced by PSAP after the confirmation of its sequence and PSAP pIRES2 EGFP and PSAP pIRES2 EGFP Csp7 eukaryotic expression vectors were obtained respectively. Lipofection mediated gene transfers were performed with the two obtained vectors and a control plasmid, pIRES2 EGFP, in human prostate cancer cell line PC 3 m and 2 other cell lines (Hep2 and MC3T3 ). Light and electron microscopy were used to observe the proliferation and apoptosis of all cell lines. A PSAP driven EGFP was also used to estimate the expression of the PSAP driven transcripts. Immunohistochemistry methods were used to evaluate the expression of caspase 7 protein. RESULTS: All the constructed vectors were verified by enzyme digestion and DNA sequencing. After transfected with pIRES2 EGFP, PC 3 m, Hep2 and MC3T3 all showed green fluorescence. After transfected with PSAP pIRES2 EGFP, only PC 3m showed green fluorescence and no apoptosis cells appeared in all the three transfected groups. After transfected with PSAP caspase7 pIRES2 EGFP, PC 3 m showed the strong growth inhibition but the other two cell lines showed no changes. No caspase 7 was detected in the Hep2 and MC3T3 cell lines after transfection. Morphologically, some of the PC 3m transfected cells manifested apoptotic features. CONCLUSION: PSAP driven pro caspase 7 gene therapy inhibits the growth of human prostate cancer cells and the cytotoxic effect is restricted to the cells of prostate origin." @default.
- W2392282229 created "2016-06-24" @default.
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- W2392282229 date "2003-01-01" @default.
- W2392282229 modified "2023-09-24" @default.
- W2392282229 title "Overexpression of prostate-specific antigen promoter-driven Pro-caspase-7 for the induction of therapeutic apoptosis in prostate cancer" @default.
- W2392282229 hasPublicationYear "2003" @default.
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