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- W2392287213 abstract "Objective To obtain the recombinant angiotensin-converting enzyme inhibitory peptide(ACEIP) with high biological activity.Methods Synthesize the gene encoding angiotensin-converting enzyme inhibitory peptide,insert into expression vector pGEX-4T-2 and transform to E.coli BL21 for expression.Purify the expressed product by Glutathione Sepharose 4B affinity chromatography and HiTrap Benzmidine FF column chromatography.Determine the expression level of GST-ACEIP fusion protein by thin layer chromatogram scanning,then analyze the protein and polypeptide contents of expressed product by BCA method,and its activity by an improved HPLC method.Results Restriction analysis,PCR and sequencing proved that the target gene was successfully cloned to expression vector pGEX-4T-2.The expression level of GST-ACEIP fusion protein reached 24.6%.The protein and polypeptide contents of the expressed product were 1.1 g/L and 0.14 g/L respectively.The inhibiting activity of expressed product to angiotension-converting enzyme was 85%.Conclusion The recombinant angiotensin-converting enzyme inhibitory peptide was successfully prepared.It laid a foundation of further development of drugs for hypertension." @default.
- W2392287213 created "2016-06-24" @default.
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- W2392287213 date "2006-01-01" @default.
- W2392287213 modified "2023-09-22" @default.
- W2392287213 title "High Expression of Recombinant Angiotensin-converting Enzyme Inhibitory Peptide in E.coli" @default.
- W2392287213 hasPublicationYear "2006" @default.
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