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- W2392300865 abstract "To understand the role and function of the Cerasus humilis phytoene synthase gene(ChPSY),the full-length coding region of ChPSY cDNA from the Cerasus humilis mature fruit was linked into the prokaryotic expression vector pET-28a(+).The recombinant plasmid pET-ChPSY was transformed into E.coli BL21(DE3) for induced expression.The analysis of SDS-PAGE demonstrated that the fusion protein(6×His-PSY) was produced at 4h after induction by 1.0 mmol/L IPTG and migrated at a size of about 45.8 kD.The optimization of IPTG concentration and induction time showed the fusion protein quantity was maximal after 6 h induction by 1.2 mmol/L IPTG.The results of Western-blot demonstrated that the fusion protein(6×His-PSY) could be recognized by anti-6×His monoclonal antibody.The research provided a basis for further study on the biological function of ChPSY gene in fruit carotenoid biosynthesis of Cerasus humilis(Bge) Sok." @default.
- W2392300865 created "2016-06-24" @default.
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- W2392300865 date "2012-01-01" @default.
- W2392300865 modified "2023-09-28" @default.
- W2392300865 title "Prokaryotic Expression of ChPSY Gene from Cerasus humilis" @default.
- W2392300865 hasPublicationYear "2012" @default.
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