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- W2392327862 abstract "Objective To establish a simple and novel method named cross priming amplification combine to nucleic acid test strip for rapid detection of Bacillus anthracis. Methods The Bacillus anthracis specific primers and probes were designed by sequence alignments, then isothermal nucleic acid amplification reactions were established and the amplified products were rapidly test by colloid gold immunochromatographic strip. The sensitivity and specificity of this method was evaluated. Results The method of cross priming amplification combine to nucleic acid test strip detected Bacillus anthracis was optimized by positive control standards plasmid with the sensitivity up to 10 pg /test, and sensitivity was 103CFU/ml for detection of Sterne strain, and no cross reaction was found to detect Bacillus subtilis, Bacillus thuringiensis, Bacillus pumilus, Bacillus cereus, Bacillus mycoides.Conclusion With high sensitivity and specificity, this method will be useful for detection of Bacillus anthracisin in the field of health quarantine and public health." @default.
- W2392327862 created "2016-06-24" @default.
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- W2392327862 date "2014-01-01" @default.
- W2392327862 modified "2023-09-27" @default.
- W2392327862 title "CPA thermostatic expansion technique for detection of Bacillus anthracis" @default.
- W2392327862 hasPublicationYear "2014" @default.
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