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- W2392391489 abstract "Objective To analyze the drug sensitivity,pathogenicity and immune protection of serotype 11 Riemerella anatipestifer(RA). Methods The drug-sensitivities of 73 RA strains were analyzed by paper strip assay. Ten 10-day-old Cherry Valley ducks were injected s.c. with 2 × 108and 2 × 107CFU / ml RA suspension respectively,0. 5 ml for each, observed for 5 d after challenge and recorded for death. RA was extracted from the livers of dead ducks,and the death rate was calculated. The isolated RA1229 strain was prepared into inactivated oil emulsion vaccine and injected s.c. into 50 7-day-old ducks,0. 3 ml for each. The ducks in various groups were challenged with the 2 × 10 7 CFU / ml fresh cultures of RAf74,RA234,RA1067,RA1156 and RA1229 14 d later,0. 5 ml for each,then observed for 5 d,recorded for death and calculated for death rate,and RA was extracted for the livers of dead ducks. Results More than 90% of the RA strains were sensitive to cefazolin,florfenicol and cefalexin. In the turn of sensitivity,other drugs were amoxicillin, ampicillin,doxycycline,aiprofloxacin,amikacin and rifampicin. The pathogenicities of 10 isolates from livers of dead ducks were similar. The death rates of ducks immunized with 1 × 108and 1 × 107CFU / ml RA were 100% and 80% respectively. RA1229 showed a slightly strong pathogenicity,with which the prepared vaccine showed a protective rate of more than 90% to Cherry Valley ducks. Conclusion RA serotype 11 was sensitive to several antibiotics and showed strong pathogenicity,of which RA1229 showed good protective immunity and might be used as a candidate strain for preparation of inactivated RA vaccine." @default.
- W2392391489 created "2016-06-24" @default.
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- W2392391489 date "2014-01-01" @default.
- W2392391489 modified "2023-09-24" @default.
- W2392391489 title "Main biological characteristics of serotype 11 Riemerella anatipestifer isolates" @default.
- W2392391489 hasPublicationYear "2014" @default.
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