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- W2392424514 abstract "Objective To develope a TaqMan Probe-Based Real-time qPCR Method for the Detection and enumeration of Escherichia coli. Methods Specific primers and probes were designed to target the ydiJ gene of Escherichia coli. Moreover, the primer taxonomic specificity and sensitivity were confirmed. We also created a Plasmid DNA Calibration standards containing a segment of the ydiJ gene.The reaction systems and conditions for PCR were optimized. Then Standard curves were created for analysis. Results In this study, these primers showed good specificity with all the strains tested. The linearity of quantitative PCR was linear from 10-1.0×108copy/μl(R2:0.990-0.999). The detection and quantification limits were estimated to be 10 and 100 copy/μl respectively, the results showed that the numbers obtained by qPCR correlated with those measured by plating, r=0.991, P0.01. Conclusion The RT-qPCR is a rapid and accurate technique for enumerating Escherichia coli in water samples." @default.
- W2392424514 created "2016-06-24" @default.
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- W2392424514 date "2014-01-01" @default.
- W2392424514 modified "2023-09-26" @default.
- W2392424514 title "Development of a TaqMan Probe-Based Real-time qPCR Method for the Detection and Enumeration of Escherichia coli" @default.
- W2392424514 hasPublicationYear "2014" @default.
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