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- W2392438498 abstract "In this contribution, we report a highly selective and sensitive method for detecting cysteine based on surface energy transfer from fluorophore to gold nanoparticles. Cation dyes, such as Rhodamine B, can be adsorbed onto the surface of citrated-gold nanoparticles through the electrostatic interaction, and the fluorescence of the Rhodamine B absorbed on the surface of the as-prepared gold nanoparticles gets quenched because of the occurrence of surface energy transfer from the fluorophore of Rhodamine B to the gold nanoparticles. With the addition of cysteine, the strong covalent combination between the mercapto group of cysteine and gold nanoparticles drives Rhodamine B molecules apart from the gold nanoparticles’ surface, which reduces the energy transfer efficiency and results in a significant increase of fluorescence of the solution. It was found that the fluorescence gets increased linearly with the concentration of cysteine ranging from 0.025 μmol/L to 4.5 μmol/L. This phenomenon allows sensitive detection of cysteine with a detection limit of 8.0 nmol/L (3σ). Other 19 kinds of natural amino acids have a weak influence on the surface energy transfer." @default.
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- W2392438498 date "2010-05-01" @default.
- W2392438498 modified "2023-09-26" @default.
- W2392438498 title "Gold nanoparticles surface energy transfer and its application to highly selective and sensitive detection of cysteine" @default.
- W2392438498 doi "https://doi.org/10.1360/zb2010-40-5-531" @default.
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