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- W2392454786 abstract "Objective To study two immobilization methods of antibodies to avian influenza viruses for a piezoelectric immunity protein chips.Methods Monoclonal antibody to avian influenza virus H5 subtype was deposited on the gold electrode′s surface of AT-cut 10 MHz quartz crystal by polyethyleneimine adhesion and glutaraldehyde cross-linking method(glutaraldehyde method) and staphylococcal protein A(SPA) method.Results By glutaraldehyde method,the linear range of H5 antigen dilution was between 1∶2-1∶40,Y=75.814-13.748X,r2=0.978 9.There was no cross-reaction to H9 antigen,and the coincidence was 91.7% compared with chick embryo separation.By SPA method,it wouldn′t detect the corresponding antigen if the antibody had been immobilized to the gold electrode first.However,the qualitative detection could be gotten by combining SPA quartz crystal with antigen-antibody compounds.Conclusions The glutaraldehyde method is applicable to establish the avian infuenza viruses piezoelectric immunity protein chip.It has better linearity.The SPA method has limitation and can′t detect the antigen directly." @default.
- W2392454786 created "2016-06-24" @default.
- W2392454786 creator A5063059754 @default.
- W2392454786 date "2010-01-01" @default.
- W2392454786 modified "2023-09-24" @default.
- W2392454786 title "Study on two immobilization methods of antibodies to avian influenza viruses for a piezoelectric immunosensor" @default.
- W2392454786 hasPublicationYear "2010" @default.
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