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- W2392489473 abstract "Objective To establish a method for the detection of mRNA transcription of SLAM as CDV receptors in different canine tissues.Methods By using GAPDH as a housekeeper,The △△Ct relative quantification method was used to detect the transcription levels of SLAM mRNAs in different canine tissues.Results The inter-assay variability(CV%) was 0.89%-2.35%.The RT-PCR assay had advantages of high reproducibility.The SLAM mRNA was transcripted at high levels in the spleen,hilar lymph node,mesenteric nodes,inguinal nodes and a low level in the urinary bladder.Conclusions The quantitative RT-PCR method is successfully established for the detection of mRNA transcription of SLAM in different canine tissues." @default.
- W2392489473 created "2016-06-24" @default.
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- W2392489473 date "2013-01-01" @default.
- W2392489473 modified "2023-09-24" @default.
- W2392489473 title "Development and application of fluorescence quantitative RT-PCR for detection of slam mRNA in canine tissues" @default.
- W2392489473 hasPublicationYear "2013" @default.
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