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- W2392497889 abstract "The anticancer rare sugar D-allose was converted from D-psicose by E. coli BL21/ p ET-22b-tl-rpib.The LC-MS/MS results showed that 25% of D-psicose was converted to D-allose in the reaction product mixture. The p I of the recombinant protein was 6.3 determined by isoelectrofocusing and was successfully applied in isoelectric precipitation. The methods of separation and purification of D-allose was established according to DTF-Ca2 +cation exchange chromatography with a flow rate of 1m L/min,column temperature at60℃ and the loading volume of 4m L." @default.
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- W2392497889 date "2014-01-01" @default.
- W2392497889 modified "2023-09-23" @default.
- W2392497889 title "Separation and purification of D-allose produced by biotransformation" @default.
- W2392497889 hasPublicationYear "2014" @default.
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