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- W2392498855 abstract "The object of this research were to develop EST-SSR markers based on ESTs from the spikes of photo-thermo sensitive genic male sterile wheat lines,and test their application in wheat genetic diversity analysis.About 3264EST sequences of PTGMS wheat spikes were screened using SSRSCAN software to search for SSR motifs,and 108SSRs were obtained.64pairs of primers were designed with the Primer 5.0Plus software and the PCR products of these primers were detected by PAGE.The results showed that among the designed 64EST-SSR primers,41amplified distinct bands and expected products in 42different wheat,and 36were polymorphic.The dendrogram showed that the genetic similarity coefficient in 42wheat cultivars was between 0.64and 0.87,and 3PTGMS wheat had a high genetic similarity coefficient.Due to high efficiency in developing SSR markers on ESTs of PTGMS wheat spikes,this method would be of great importance in identification of wheat varieties and wheat genetic analysis,and as the specific ESTs from wheat spikes,the development of EST-SSR markers might be a great help on study of wheat flower development,the yield traits,promoting the hybrid wheat breeding." @default.
- W2392498855 created "2016-06-24" @default.
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- W2392498855 date "2013-01-01" @default.
- W2392498855 modified "2023-09-28" @default.
- W2392498855 title "Development of SSR Markers Derived from EST of Wheat Spikes and Their Use in Genetic Diversity Analysis of Wheat" @default.
- W2392498855 hasPublicationYear "2013" @default.
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