Matches in SemOpenAlex for { <https://semopenalex.org/work/W2392503886> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2392503886 abstract "Twenty one pairs of microsatellite primers developed from shovelnose sturgeon ( Scaphirhynchus platorynchus ) were tested on Chinese sturgeon ( Acipenser sinensis ). About 67% (14 loci) of the S.platorynchus primers amplified successfully with 48% (10 loci) being polymorphic. However, only 9 5% (2 loci) of these primers (Spl 100 Spl 168) produced clear and sharp products without stutter bands. Several amplified fragments produced by four pairs of the rest primers, which generated stutter bands in Chinese sturgeon, were sequenced. One of these four loci (Spl 123) was proved without repeat motif. Then, the other three, Spl 106,Spl 163, and Spl 170α, were redesigned with Primer premier 5 0 software and renamed as Spl 106a, Spl 163a, and Spl 170b. Further amplifications using these primers in Chinese sturgeon showed that Spl 106a and Spl 170b could give clear and polymorphic bands without any stutter, but Spl 163a did not get any bands. Moreover, motif sequences obtained by both Spl 106 and Spl 106a in Chinese sturgeon are the same but differ to those in shovelnose sturgeon. It seems that the redesigned primer Spl 106a could be a species specific primer for Chinese sturgeon, and we finally renamed it as As 100. This result indicates that it is viable to obtain species specific microsatellite primers by redesigning from the primers of related species." @default.
- W2392503886 created "2016-06-24" @default.
- W2392503886 creator A5004548899 @default.
- W2392503886 date "2002-01-01" @default.
- W2392503886 modified "2023-09-25" @default.
- W2392503886 title "APPLICABILITY OF MICROSATELLITE PRIMERS DEVELOPED FROM SHOVELNOSE STURGEON IN CHINESE STURGEON" @default.
- W2392503886 hasPublicationYear "2002" @default.
- W2392503886 type Work @default.
- W2392503886 sameAs 2392503886 @default.
- W2392503886 citedByCount "0" @default.
- W2392503886 crossrefType "journal-article" @default.
- W2392503886 hasAuthorship W2392503886A5004548899 @default.
- W2392503886 hasConcept C104317684 @default.
- W2392503886 hasConcept C178790620 @default.
- W2392503886 hasConcept C180754005 @default.
- W2392503886 hasConcept C185592680 @default.
- W2392503886 hasConcept C2777563447 @default.
- W2392503886 hasConcept C2777784394 @default.
- W2392503886 hasConcept C2909208804 @default.
- W2392503886 hasConcept C505870484 @default.
- W2392503886 hasConcept C54355233 @default.
- W2392503886 hasConcept C61320498 @default.
- W2392503886 hasConcept C86803240 @default.
- W2392503886 hasConcept C90856448 @default.
- W2392503886 hasConceptScore W2392503886C104317684 @default.
- W2392503886 hasConceptScore W2392503886C178790620 @default.
- W2392503886 hasConceptScore W2392503886C180754005 @default.
- W2392503886 hasConceptScore W2392503886C185592680 @default.
- W2392503886 hasConceptScore W2392503886C2777563447 @default.
- W2392503886 hasConceptScore W2392503886C2777784394 @default.
- W2392503886 hasConceptScore W2392503886C2909208804 @default.
- W2392503886 hasConceptScore W2392503886C505870484 @default.
- W2392503886 hasConceptScore W2392503886C54355233 @default.
- W2392503886 hasConceptScore W2392503886C61320498 @default.
- W2392503886 hasConceptScore W2392503886C86803240 @default.
- W2392503886 hasConceptScore W2392503886C90856448 @default.
- W2392503886 hasLocation W23925038861 @default.
- W2392503886 hasOpenAccess W2392503886 @default.
- W2392503886 hasPrimaryLocation W23925038861 @default.
- W2392503886 hasRelatedWork W1439753198 @default.
- W2392503886 hasRelatedWork W1992128517 @default.
- W2392503886 hasRelatedWork W2184707787 @default.
- W2392503886 hasRelatedWork W2218850527 @default.
- W2392503886 hasRelatedWork W2351971048 @default.
- W2392503886 hasRelatedWork W2357707800 @default.
- W2392503886 hasRelatedWork W2357970869 @default.
- W2392503886 hasRelatedWork W2359151925 @default.
- W2392503886 hasRelatedWork W2359724153 @default.
- W2392503886 hasRelatedWork W2364710453 @default.
- W2392503886 hasRelatedWork W2372650167 @default.
- W2392503886 hasRelatedWork W2373474263 @default.
- W2392503886 hasRelatedWork W2376104815 @default.
- W2392503886 hasRelatedWork W2378149995 @default.
- W2392503886 hasRelatedWork W2383539071 @default.
- W2392503886 hasRelatedWork W2391345567 @default.
- W2392503886 hasRelatedWork W2994769113 @default.
- W2392503886 hasRelatedWork W2829319610 @default.
- W2392503886 hasRelatedWork W2961713338 @default.
- W2392503886 hasRelatedWork W3017787100 @default.
- W2392503886 isParatext "false" @default.
- W2392503886 isRetracted "false" @default.
- W2392503886 magId "2392503886" @default.
- W2392503886 workType "article" @default.