Matches in SemOpenAlex for { <https://semopenalex.org/work/W2392506252> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2392506252 endingPage "417" @default.
- W2392506252 startingPage "412" @default.
- W2392506252 abstract "To construct and express fusion protein of HLA-A*0206-BSP and-A*0207-BSP,the full-length HLA-A*0206,-A*0207 cDNA was amplified from peripheral blood mononuclear cells of an HLA-A*0206 positive donor and an HLA-A*0207 positive donor,respectively,by RT-PCR with HLA-A2 locus-specific primers.The cDNA was recombined into plasmid pcDNA3.1 to form pcDNA-HLA-A*0206 and pcDNA-HLA-A*0207.The extracellular fractions of HLA-A*0206,-A*0207(without encoding signal peptide and the fragment of transmembrane) were cloned by PCR using pcDNA-HLA-A*0206 or pcDNA-HLA-A*0207 as template.These sequences of HLA-A*0206(extra) and-A*0207(extra) were used to replace the HLA-A*0201 sequence in a pre-existing HLA-A*0201-BSP vector,to generate an HLA-A*0206-BSP and an HLA-A*0207-BSP expressing vectors.After transformation of E.coli BL21(DE3) with the vectors,the fusion protein was expressed and purified.The fusion protein was refolded in vitro by limiting dilution with β2m and HLA-A2 restricted peptide(HBc18-27 NH2-FLPSDFFPSV-COOH) to produce the sHLA-A*0206-peptide complex monomer.The conformation of the monomer was verified by ELISA and Western blotting using HLA class I specific mAb w6/32 or/and β2m-specific antibody.Restriction enzyme assay and DNA sequencing showed the HLA-A*0206-BSP and-A*0207-BSP was constructed,which could be expressed in E.coli after transformation.The HLA-A*0206-BSP and-A*0207-BSP fusion protein can be refolded with β2m and peptide in vitro into peptide/HLA-A*0206 or HLA-A*0207 complex monomer,as the ELISA and Western blotting showed the monomer share the natural conformation with classical HLA class I molecule.High efficient expression of HLA-A*0206 or HLA-A*0207 fusion protein lays the foundation for the constructing MHC-peptide tetramers and artificial antigen presenting cells to explore the allelic feature of corresponding HLA-A2 subtype in T-cell recognition." @default.
- W2392506252 created "2016-06-24" @default.
- W2392506252 creator A5003826598 @default.
- W2392506252 creator A5022321727 @default.
- W2392506252 creator A5026856551 @default.
- W2392506252 creator A5050648646 @default.
- W2392506252 creator A5054678559 @default.
- W2392506252 creator A5077988536 @default.
- W2392506252 creator A5081663262 @default.
- W2392506252 date "2007-01-01" @default.
- W2392506252 modified "2023-09-23" @default.
- W2392506252 title "Construction and expression of HLA-A*0206-BSP and-A*0207-BSP fusion genes" @default.
- W2392506252 hasPublicationYear "2007" @default.
- W2392506252 type Work @default.
- W2392506252 sameAs 2392506252 @default.
- W2392506252 citedByCount "0" @default.
- W2392506252 crossrefType "journal-article" @default.
- W2392506252 hasAuthorship W2392506252A5003826598 @default.
- W2392506252 hasAuthorship W2392506252A5022321727 @default.
- W2392506252 hasAuthorship W2392506252A5026856551 @default.
- W2392506252 hasAuthorship W2392506252A5050648646 @default.
- W2392506252 hasAuthorship W2392506252A5054678559 @default.
- W2392506252 hasAuthorship W2392506252A5077988536 @default.
- W2392506252 hasAuthorship W2392506252A5081663262 @default.
- W2392506252 hasConcept C104317684 @default.
- W2392506252 hasConcept C123894998 @default.
- W2392506252 hasConcept C147483822 @default.
- W2392506252 hasConcept C153911025 @default.
- W2392506252 hasConcept C187882448 @default.
- W2392506252 hasConcept C188280979 @default.
- W2392506252 hasConcept C40767141 @default.
- W2392506252 hasConcept C54355233 @default.
- W2392506252 hasConcept C86803240 @default.
- W2392506252 hasConceptScore W2392506252C104317684 @default.
- W2392506252 hasConceptScore W2392506252C123894998 @default.
- W2392506252 hasConceptScore W2392506252C147483822 @default.
- W2392506252 hasConceptScore W2392506252C153911025 @default.
- W2392506252 hasConceptScore W2392506252C187882448 @default.
- W2392506252 hasConceptScore W2392506252C188280979 @default.
- W2392506252 hasConceptScore W2392506252C40767141 @default.
- W2392506252 hasConceptScore W2392506252C54355233 @default.
- W2392506252 hasConceptScore W2392506252C86803240 @default.
- W2392506252 hasOpenAccess W2392506252 @default.
- W2392506252 hasRelatedWork W2233781908 @default.
- W2392506252 hasRelatedWork W2347299188 @default.
- W2392506252 hasRelatedWork W2358393024 @default.
- W2392506252 hasRelatedWork W2362235249 @default.
- W2392506252 hasRelatedWork W2364017788 @default.
- W2392506252 hasRelatedWork W2368672550 @default.
- W2392506252 hasRelatedWork W2370397273 @default.
- W2392506252 hasRelatedWork W2372809259 @default.
- W2392506252 hasRelatedWork W2372927515 @default.
- W2392506252 hasRelatedWork W2373018528 @default.
- W2392506252 hasRelatedWork W2374007278 @default.
- W2392506252 hasRelatedWork W2375308865 @default.
- W2392506252 hasRelatedWork W2375914389 @default.
- W2392506252 hasRelatedWork W2381385267 @default.
- W2392506252 hasRelatedWork W2386935393 @default.
- W2392506252 hasRelatedWork W2387360189 @default.
- W2392506252 hasRelatedWork W2388046372 @default.
- W2392506252 hasRelatedWork W2389846048 @default.
- W2392506252 hasRelatedWork W274695362 @default.
- W2392506252 hasRelatedWork W3148645544 @default.
- W2392506252 isParatext "false" @default.
- W2392506252 isRetracted "false" @default.
- W2392506252 magId "2392506252" @default.
- W2392506252 workType "article" @default.