Matches in SemOpenAlex for { <https://semopenalex.org/work/W2392571057> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2392571057 abstract "[Objective]The aim was to lay the foundation for the further study of population genetic diversity of Cybidium goeringii from the molecular level.[Method]With the genomic DNA of C.goeringii as template,the influence of the main components in the ISSR reaction system on the amplification result was studied by single factor experiment to search for the reaction system suitable for ISSR analysis of C.goeringii.[Result]The suitable amplification conditions of ISSR reaction system in C.goeringii was as 15.78 μl double distilled water,2.5 μl 1×PCR buffer,1.1 U Taq DNA polymerase,100 ng genomic DNA,2.0 mmol/L MgCl2,150 μmol/L dNTPs,2 μmol/L primer in the 25 μl PCR reaction volume.The optimum amplification programme was that after pre-denaturing of 5 min at 94 ℃,40 cycles were performed with denaturing of 45 s at 94 ℃,denaturing temperature of 1～2 ℃ lower than the different primer's TM value,annealing of 45 s,extension of 75 s at 72 ℃,a final extension step of 7 min at 72 ℃ and preservation at 4 ℃.[Conclusion] The establishment of the optimization system laid the standardization program for the identification of C.goeringii and the analysis of its gemplasm genetic diversity." @default.
- W2392571057 created "2016-06-24" @default.
- W2392571057 creator A5083416163 @default.
- W2392571057 date "2008-01-01" @default.
- W2392571057 modified "2023-09-26" @default.
- W2392571057 title "Establishment and Optimization of ISSR-PCR Reaction System for Cybidium goeringii" @default.
- W2392571057 hasPublicationYear "2008" @default.
- W2392571057 type Work @default.
- W2392571057 sameAs 2392571057 @default.
- W2392571057 citedByCount "0" @default.
- W2392571057 crossrefType "journal-article" @default.
- W2392571057 hasAuthorship W2392571057A5083416163 @default.
- W2392571057 hasConcept C104317684 @default.
- W2392571057 hasConcept C144024400 @default.
- W2392571057 hasConcept C149923435 @default.
- W2392571057 hasConcept C153911025 @default.
- W2392571057 hasConcept C17757408 @default.
- W2392571057 hasConcept C178790620 @default.
- W2392571057 hasConcept C185592680 @default.
- W2392571057 hasConcept C2756471 @default.
- W2392571057 hasConcept C2777563447 @default.
- W2392571057 hasConcept C2779186596 @default.
- W2392571057 hasConcept C2781440490 @default.
- W2392571057 hasConcept C2908647359 @default.
- W2392571057 hasConcept C49105822 @default.
- W2392571057 hasConcept C54355233 @default.
- W2392571057 hasConcept C552990157 @default.
- W2392571057 hasConcept C81977670 @default.
- W2392571057 hasConcept C86803240 @default.
- W2392571057 hasConceptScore W2392571057C104317684 @default.
- W2392571057 hasConceptScore W2392571057C144024400 @default.
- W2392571057 hasConceptScore W2392571057C149923435 @default.
- W2392571057 hasConceptScore W2392571057C153911025 @default.
- W2392571057 hasConceptScore W2392571057C17757408 @default.
- W2392571057 hasConceptScore W2392571057C178790620 @default.
- W2392571057 hasConceptScore W2392571057C185592680 @default.
- W2392571057 hasConceptScore W2392571057C2756471 @default.
- W2392571057 hasConceptScore W2392571057C2777563447 @default.
- W2392571057 hasConceptScore W2392571057C2779186596 @default.
- W2392571057 hasConceptScore W2392571057C2781440490 @default.
- W2392571057 hasConceptScore W2392571057C2908647359 @default.
- W2392571057 hasConceptScore W2392571057C49105822 @default.
- W2392571057 hasConceptScore W2392571057C54355233 @default.
- W2392571057 hasConceptScore W2392571057C552990157 @default.
- W2392571057 hasConceptScore W2392571057C81977670 @default.
- W2392571057 hasConceptScore W2392571057C86803240 @default.
- W2392571057 hasLocation W23925710571 @default.
- W2392571057 hasOpenAccess W2392571057 @default.
- W2392571057 hasPrimaryLocation W23925710571 @default.
- W2392571057 hasRelatedWork W1420062932 @default.
- W2392571057 hasRelatedWork W2351342401 @default.
- W2392571057 hasRelatedWork W2351705354 @default.
- W2392571057 hasRelatedWork W2550670369 @default.
- W2392571057 hasRelatedWork W2807922880 @default.
- W2392571057 hasRelatedWork W2924492604 @default.
- W2392571057 hasRelatedWork W2944224831 @default.
- W2392571057 hasRelatedWork W2977738773 @default.
- W2392571057 hasRelatedWork W3141743346 @default.
- W2392571057 hasRelatedWork W3142406879 @default.
- W2392571057 hasRelatedWork W3142807146 @default.
- W2392571057 hasRelatedWork W3144433127 @default.
- W2392571057 hasRelatedWork W3147264161 @default.
- W2392571057 hasRelatedWork W3147571025 @default.
- W2392571057 hasRelatedWork W3147698144 @default.
- W2392571057 hasRelatedWork W3148336909 @default.
- W2392571057 hasRelatedWork W3150547096 @default.
- W2392571057 hasRelatedWork W2929270213 @default.
- W2392571057 hasRelatedWork W2934558046 @default.
- W2392571057 hasRelatedWork W3001687535 @default.
- W2392571057 isParatext "false" @default.
- W2392571057 isRetracted "false" @default.
- W2392571057 magId "2392571057" @default.
- W2392571057 workType "article" @default.