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- W2392572514 abstract "AIM: To explore the angiogenesis induced by uraemic sera medium in vitro and its mechanism.METHODS: Human unbilical vein endothelial cells(HUVECs) were respectively cultured in 100 mL/L haemodialysis patients sera medium(experimental group) and 100 mL/L healthy persons sera medium(control group).Vascular structure formation was observed under light microscope.Cell proliferation was examinded by MTT.Cell migration was measured in transwell chamber,ROS(reactive oxygen species) was investigated by spectrophotometry and expression of p-p38 protein was evaluated by Western Blot.RESULTS: After HUVECs were treated for 30 hours in experimental group,the average vascular structure area was(1438±240) μm2,mean optical absorption value was 1.073±0.046 in MTT,and average number of migration cells was 63.7±4.5,all of which were significantly different compared with control group.The expression of p-p38 protein and ROS increased respectively at 10 hours and 30 hours.CONCLUSION: Uraemic sera medium can induce angiogenesis in vitro,and the activation of p-p38 and ROS may be involved in the mechanism." @default.
- W2392572514 created "2016-06-24" @default.
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- W2392572514 date "2008-01-01" @default.
- W2392572514 modified "2023-09-24" @default.
- W2392572514 title "In vitro induction of angiogenesis with sera medium of maintenance hemodialysis patients" @default.
- W2392572514 hasPublicationYear "2008" @default.
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