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- W2392604674 abstract "A pair of primers were designed according to the Genebank-reported complete genome of duck hepatitis virusⅠ(DHV-Ⅰ),and a specific gene segment of DHV-Ⅰwas obtained by RT-PCR.The segment was cloned into the expression vector pET-28a,then the recombinant plasmids were transfected into Rosetta-gami(DE3),induced by IPTG and purified by the Ni-NTA,and thus the recombinant fusion protein(20 kD or so)was obtained.The BALB/c mice were immunized with this recombinant protein,and a monoclonal antibody cell strain of DHV-Ⅰwas produced.The Western-blot analysis and ELISA showed that the recombinant protein of DHV-Ⅰ could react with DHV-Ⅰpositive serum and DHV-Ⅰmonoclonal antibody.Also the DHV-Ⅰmonoclonal antibody could detect natural protein of DHV-Ⅰthrough western-blot assay." @default.
- W2392604674 created "2016-06-24" @default.
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- W2392604674 date "2012-01-01" @default.
- W2392604674 modified "2023-09-28" @default.
- W2392604674 title "Prokaryotic expression of gene segment of duck hepatitis virusI and preparation of monoclonal antibody of DHV-I" @default.
- W2392604674 hasPublicationYear "2012" @default.
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