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- W2392606488 abstract "Objective To explore the mechanism of signal cascades in hyperosmolarity-induced mucous hypersecretion.Methods NCl-H292 airway epithelial cells were cultured in 545,695,855,1 055 and 1 280 mOsm/L hypertonic saline solution.At the same time,1 280 mOsm/L hypertonic saline was uesd as a stimulus to P38 inhibitor SB203580,c-jun N-terminal kinase(JNK) inhibitor SP600125 and extracellular signal-regulated protein kinase(ERK)-specific inhibitor U0126 were uesd as the intervention factors.The transcription levels of MUC5AC and MUC5AC protein levels in the cultured cells were observed with RT-PCR technology and ELISA.The cell homogenate phosphorylated P38(p-P38),phosphorylated JNK(p-JNK) and phosphorylated ERK(p-ERK) protein level in 1 280 mOsm/L hypertonic saline supernatant were determined by Western blotting.Results After the stimulation by 545,695,855,1 055 and 1 280 mOsm/L hypertonic saline respectively,the expression of MUC5AC mRNA and protein contents in cultured cells were significantly higher than those of control group(P0.01),which were associated with the exposure doses.SB203580 significantly reduced the increase of p-P38 levels induced by 1 280 mOsm/L hypertonic saline,while significantly reduced the expression of MUC5AC mRNA and protein content(P0.05);SP600125 markedly reduced the levels of pJNK,transcription of MUC5AC mRNA and protein content(P0.05),but its effects were weaker than SB203580;1 280 mOsm/L hypertonic saline had no effect on ERK levels(P0.05),U0126 had no significant effects on MUC5AC mRNA transcription and protein content also(P0.05).Conclusion Hypertonic stress can induce the airway epithelial cells to develop a high mucus secretion with the concentration-dependent manner in the level of transcription,P38 signal pathway plays a major role." @default.
- W2392606488 created "2016-06-24" @default.
- W2392606488 creator A5023217183 @default.
- W2392606488 date "2010-01-01" @default.
- W2392606488 modified "2023-09-23" @default.
- W2392606488 title "P38 Signal Cascades in Hyperosmolarity-Induced Mucous Hypersecretion" @default.
- W2392606488 hasPublicationYear "2010" @default.
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