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- W2392650663 abstract "The recent opportunity of examining the faecal material of the water-scorpion Leccotrephes japonensis revealed a flagellate which had not previously been described from this host. After fuller investigation I found that it is a species new to science and should be referred to the genus Retortamona; I have named it Retortamonas leccotrephae sp. nov.The specimens of Leccotrephes japonensis used for studies on the parasitic flagellate R. leccotrephae, were collected from ponds on the outskirts of Shanghai, China. Apart from studying the living flagellates, at the same time stained specimens were prepared for the detailed study of various structures. These specimens were fixed either in Hollande's or in Schaudinn's fluid; stained in Heidenhain's haematoxylin, and destained in 2% aqueous phospho-tungstic acid. Moskowitz' (1950) modification of the Protargol impregnation method was also used for staining.In the living state, the flagellare has a slender, slipper-shaped trunk with an anterior dorsal flexure. The mean body size, exclusive of the posterior spike, is 12.8μ×4.8μ. The length of the slender posterior spike varies from 4.6μ to 15.8μ. There are two flagella of unequal length. The longer one extends forwards and lashes rapidly, causing the animal to rotate and to pursue a special course. The shorter flagellum is directed backwards and usually lies in the cytostome; it moves in an undulating manner.In fixed and stained preparations, the flagellates, not including the posterior spike, show a size-range of 6.2×2.2μto 15.6×5.4μ, with a mean of 11.9×3.4μ. The spike varied from 4.6μ to 13.6μ long, with an average length of 11.2μ. In general, a normal specimen of R. leccotrephae has a slender body; the anterior portion is narrower than the postcerior, and is markedly bent as shown in the living indivi.duals. The posterior portion of the body usually has a dorsal convexity, and the widest region is located near the two-thirds of the body-length from the anterior end.Near that edge of the nuclear membrane lying next to the cytostome, there are two minute, but separate, basal granules which give rise to the two flagella. The anterior flagellum is about as long as the body proper: much longer and more slender than the posterior flagellum, which usually lies within the cytostome, and commonly shows a series of two or three undulations; it often stains more deeply than the anterior flagellum.In hematoxylin preparations the nucleus is well stained, and clearly shows a layer of chromatin granules lying against the nuclear membrane, and a large central endosome, composed of a mass of granules.The cytostome is a large structure in this animal. It usually occupies about twothirds of the total length of the body proper. Along the margin of the cytostome there are two deeply staining fibres, the one on the right margin is longer than that on the left.Both the precystic forms and cysts of R. leccotrephae have been observed. The body of precystic individuals which are preparing to encyst, becomes smailer and rounds up; meanwhile, the chromatin of the nucleus condenses and forms a deeply staining endosome. The anterior flagellum is still disposed freely, and no cyst wall has as yet been formed. The border-line of the cytostome is distinct, but the two basal granules are visible only in favourable preparations. The mature cysts are nearly peanut-shaped, and are about twice as long as wide. In the stained specimens they are about 4.6μ long and 2.4μ wide. The cyst wail is of uniform thickness, and the nucleus is visible at one end. The other conspicuous structures within the cyst are the two flagella and the cytostomial fibres, which are arranged as they are in the trophozoite, except that the anterior flagellum is here directed posteriorly.DISCUSSION SUMMARYIn its general body-form, R. leccotrephae closely resembles Mackinnon's R. agilis ('11) from crane-fly larvae, and also Corradett's R. gryllotalpae ('37) from the mole cricket, but it is especially like Geiman's R. caudacus ('32) from the aquatic larvae ef certain beetles." @default.
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- W2392650663 date "1953-01-01" @default.
- W2392650663 modified "2023-09-26" @default.
- W2392650663 title "A NEW SPECIES OF RETORTAMONAS (PROTOZOA: FLAGELLATA) FROM THE WATER SCORPION, LECCOTREPHES JAPONENSIS" @default.
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