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- W2392655451 abstract "Objective To study the effects of proliferated activity of three-dimensional cultured fibroblasts in different dose and at different time.Methods The rat tail collagen were prepared the three-dimensional scaffold,fibroblasts(L929 cell line) were cultured and planted in the three-dimensional scaffold,fibroblasts was divided into control group and 6 group of fluoride(0.001,0.002,0.1,1,10and 20 mg/L F-),MTT colorimetry was used to detect effects of fluoride on fibroblast proliferated activity after existence of fluoride 1,2,4,24,48,72 and 96 h.Results Fluoride increased the proliferated activity of fibroblasts in certain group(1 h and 2 h 0.002 mg/L,2 h 0.1 mg/L).The proliferation of fibroblasts was increased in the initial stage as the existence of fluoride,the others was decreased or obviously decreased ;Fluoride increased the proliferated activity of fibroblasts than the control group at certain time(24,48,72 h).but the results didn't reach the significant level of statistics.Conclusions Fluoride increased proliferated activity of the three-dimensional cultured fibroblasts,nevertheless,the effect was fairly slow,the degree was relatively feeble." @default.
- W2392655451 created "2016-06-24" @default.
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- W2392655451 date "2008-01-01" @default.
- W2392655451 modified "2023-09-27" @default.
- W2392655451 title "Effects of fluoride on proliferated activity of three-dimensional cultured fibroblasts" @default.
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