Matches in SemOpenAlex for { <https://semopenalex.org/work/W2392667780> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2392667780 abstract "Objective To study the effects of reduced glutathione(GSH)on doxorubicin(DOX)induced proliferation and apoptosis in HepG2,and to investigate the expression of NF-κB p65,Bcl-2 in apoptostic HepG2 induced by DOX monotherapy and DOX combination with GSH,respectively.Methods Four groups were devided,including blank group:RPMl l640 medium only;control group:HepG2 cells were cultured in the same volume of medium;DOX group:cells were cultured in medium containing DOX;DOX+GSH group:cells were cultured in medium containing DOX and GSH.MTT assay was performed to probe proliferation of HepG2 cell.Flow cytometry was carried out to determine early apoptosis rate of HepG2 cells.Real-time polymerase chain reaction was administered to investigate NF-κB p65 mRNA expression.Western blotting was used to document protein expression of NF-κB p65 and Bcl-2.The experimental data was analyzed by SPSS version 19.0software.Results(1)HepG2 cell proliferation was inhibited time-and dose-dependently in both DOX group and DOX + GSH group at 24 h or 48 h or 72 h.Additionally,the proliferation rate was higher in DOX group than that in DOX+GSH group(P0.05).(2)The apoptosis rate of HepG2 was(0.733±0.153)%in control group,(28.400±0.007)%in DOX group,(15.500±0.006)%in DOX+GSH group,respectively,which showed statistically significant difference between the later two groups and control group(P0.01),as well as between DOX group and DOX+ GSH group(P0.01).(3)The mRNA expression of NF-κB p65 in DOX+GSH group was higher than that in DOX group(P0.05).(4)The protein expression of NF-κB p65 and Bcl-2in DOX+GSH group was higher than that in DOX group(P0.05).Conclusion DOX and GSH combination therapy could attenuate chemotherapeutic effect though a potential mechanism by increasing expression of NF-κB p65 and Bcl-2.The combination of DOX and GSH in chemotherapy for cancer patients should be avoided." @default.
- W2392667780 created "2016-06-24" @default.
- W2392667780 creator A5056757809 @default.
- W2392667780 date "2015-01-01" @default.
- W2392667780 modified "2023-09-27" @default.
- W2392667780 title "Effects of reduced glutathione against doxorubicin in HepG2 cell line" @default.
- W2392667780 hasPublicationYear "2015" @default.
- W2392667780 type Work @default.
- W2392667780 sameAs 2392667780 @default.
- W2392667780 citedByCount "0" @default.
- W2392667780 crossrefType "journal-article" @default.
- W2392667780 hasAuthorship W2392667780A5056757809 @default.
- W2392667780 hasConcept C104317684 @default.
- W2392667780 hasConcept C153911025 @default.
- W2392667780 hasConcept C181199279 @default.
- W2392667780 hasConcept C185592680 @default.
- W2392667780 hasConcept C190283241 @default.
- W2392667780 hasConcept C2776694085 @default.
- W2392667780 hasConcept C2780783641 @default.
- W2392667780 hasConcept C2781303535 @default.
- W2392667780 hasConcept C538909803 @default.
- W2392667780 hasConcept C54355233 @default.
- W2392667780 hasConcept C553184892 @default.
- W2392667780 hasConcept C55493867 @default.
- W2392667780 hasConcept C62112901 @default.
- W2392667780 hasConcept C81885089 @default.
- W2392667780 hasConcept C85265743 @default.
- W2392667780 hasConcept C86803240 @default.
- W2392667780 hasConcept C98274493 @default.
- W2392667780 hasConceptScore W2392667780C104317684 @default.
- W2392667780 hasConceptScore W2392667780C153911025 @default.
- W2392667780 hasConceptScore W2392667780C181199279 @default.
- W2392667780 hasConceptScore W2392667780C185592680 @default.
- W2392667780 hasConceptScore W2392667780C190283241 @default.
- W2392667780 hasConceptScore W2392667780C2776694085 @default.
- W2392667780 hasConceptScore W2392667780C2780783641 @default.
- W2392667780 hasConceptScore W2392667780C2781303535 @default.
- W2392667780 hasConceptScore W2392667780C538909803 @default.
- W2392667780 hasConceptScore W2392667780C54355233 @default.
- W2392667780 hasConceptScore W2392667780C553184892 @default.
- W2392667780 hasConceptScore W2392667780C55493867 @default.
- W2392667780 hasConceptScore W2392667780C62112901 @default.
- W2392667780 hasConceptScore W2392667780C81885089 @default.
- W2392667780 hasConceptScore W2392667780C85265743 @default.
- W2392667780 hasConceptScore W2392667780C86803240 @default.
- W2392667780 hasConceptScore W2392667780C98274493 @default.
- W2392667780 hasOpenAccess W2392667780 @default.
- W2392667780 hasRelatedWork W1480561111 @default.
- W2392667780 hasRelatedWork W1966241148 @default.
- W2392667780 hasRelatedWork W2034780299 @default.
- W2392667780 hasRelatedWork W2082436593 @default.
- W2392667780 hasRelatedWork W2153454059 @default.
- W2392667780 hasRelatedWork W2322141481 @default.
- W2392667780 hasRelatedWork W2361953226 @default.
- W2392667780 hasRelatedWork W2401206126 @default.
- W2392667780 hasRelatedWork W2414440289 @default.
- W2392667780 hasRelatedWork W2415650326 @default.
- W2392667780 hasRelatedWork W2419566382 @default.
- W2392667780 hasRelatedWork W2444304796 @default.
- W2392667780 hasRelatedWork W2460035033 @default.
- W2392667780 hasRelatedWork W2896303756 @default.
- W2392667780 hasRelatedWork W2980987393 @default.
- W2392667780 hasRelatedWork W3004160938 @default.
- W2392667780 hasRelatedWork W3031969014 @default.
- W2392667780 hasRelatedWork W3035059494 @default.
- W2392667780 hasRelatedWork W3200517152 @default.
- W2392667780 hasRelatedWork W1648648517 @default.
- W2392667780 isParatext "false" @default.
- W2392667780 isRetracted "false" @default.
- W2392667780 magId "2392667780" @default.
- W2392667780 workType "article" @default.