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- W2392669837 abstract "The dynamic distribution of three different glycoconjugates in oocytesand follicle cells during the oogenesis of Atractomorpha sinensis were detected using biotin-labeled Peanut Agglutinin (PNA), Soy BeanAgglutinin (SBA) and Ulex Europaeus Agglutinin I (UEA-I) lectins. Theresults showed that during oogenesis there was no distribution of theUEA-I receptor. The receptors of PNA and SBA were found to be dependenton developmental stage and present different distribution patternsaccordingly. The binding sites of the two lectins indicated thepresence of different sugars (PNA for Galβ1,3GalNAc and SBA forGalNAc) and showed considerable variation during oogenesis. PNA and SBAreceptors first appeared at the oocyte growth phase, the PNA receptorsthen disappeared gradually and the SBA receptors exhibited the greatestexpression. At the early phase of yolk formation, PNA and SBA receptorswere located just at the brim of ooplasm, which was the region ofvitellin formation. However at the later phase of yolk formation,neither of the two receptors was detected. In the mature egg, PNA andSBA receptors were distributed again on the vitellin membrane and theeggshell. The two receptors were also widely distributed in thefollicular cells, showing similar distribution variation to theoocytes. The results indicate that the change and modification of thetwo receptors may be greatly related to the growth of oocytes, thepreparation for yolk formation, the differentiation of follicular cellsand the maturation of oocytes. The glycoconjugates on the vitellinmembrane probably play important roles in sperm and egg recognition.The two lectins bound moderately or strongly to the eggshell, whichindicates that the eggshell of A. sinensis contains the GalNAc andGalβ1,3GalNAc glycoproteins." @default.
- W2392669837 created "2016-06-24" @default.
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- W2392669837 date "2006-12-22" @default.
- W2392669837 modified "2023-09-28" @default.
- W2392669837 title "Dynamic Distribution of Glycoconjugates During Oogenesis of Atractomorpha sinensis" @default.
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