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- W2392676965 abstract "Objective:To explore the interaction of CCK-8 and endogenous opioid system in the development of opioid dependence and its mechanism,and observed the effects of different doses of CCK-8 on endogenous opioid system of morphine dependent SH-SY5Y cells. Methods:The SH-SY5Y cell model of chronic morphine dependence was established. The binding characteristic of μ opioid receptor (MOR) and expression of PENK and POMC gene were studied by radio-ligand binding assay and real-time PCR,respectively. Also,the effects of CCK-8 on above factors were investigated. Results:(1)After 10 μmol·L-1 morphine incubation for 48 h on 6 days RA differentiated SH-SY5Y cells,the cAMP content in SH-SY5Y cells was as 2.11±s 0.50 times as the level 15 min before naloxone precipitation,indicating that the cell model of chronic morphine dependence was successfully established. (2)The binding characteristics of MOR in 6 days RA differentiated SH-SY5Y cells was as follows,Bmax 378.3 pmol·mg-1±s 48.9 pmol·mg-1 Pro and Kd 0.79±s 0.09. 10-10-10-6 mol·L-1 CCK-8 suppressed dose-dependently the binding of MOR(P0.01),and this effect could be reversed by CCK1 and CCK2 receptor antagonist(P0.01). (3)The expression of PENK and POMC was down-regulated to a level of 0.43±s 0.10 and 0.37±s 0.07 times as control group by 10 μmol·L-1 morphine incubation for 48 h. 10-7 and 10-6 mol·L-1 CCK-8 increased the expression of PENK and POMC in SH-SY5Y cells to a level of 5.81±s 0.84,7.26±s1.55 and 4.55±s 0.73,5.16±s 0.82 times as control group (P0.01). 10-8,10-7 and 10-6 mol·L-1 CCK-8 up-regulated the deceased PENK and POMC expression after morphine dependence,the relative expression of PENK was 0.63±s 0.10,0.86±s 0.21 and 1.17±s 0.19 vs control group(P0.01),and that of POMC was 0.46±s 0.10、0.60±s 0.11、0.96±s 0.11 vs. control group(P0.01). In addition,10-10 and 10-9mol·L-1 CCK-8 had no effect on PENK and POMC expression (P0.05). Conclusion:10-10,10-9mol·L-1 CCK-8 could suppress μ opioid receptor by activating CCK receptor,but no effect on PENK,POMC gene expression. 10-8-10-6mol·L-1 CCK-8 could improve endogenous opioid imbalance through increasing endogenous opioid peptide release." @default.
- W2392676965 created "2016-06-24" @default.
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- W2392676965 date "2010-01-01" @default.
- W2392676965 modified "2023-09-28" @default.
- W2392676965 title "Interaction of CCK-8 and endogenous opioid system in opioid dependence" @default.
- W2392676965 hasPublicationYear "2010" @default.
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