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- W2392695087 abstract "Previous research revealed that distortion is detected in transient voltage signal recorded with traditional patch clamp amplifier under current clamp mode, which is essentially resulted by electronic design of the headstage of the patch clamp. A new kind of headstage is designed to modify the defect, the circuit of which not only measures the transient potentials as the classical voltage follower does but also is quite suitable for the standard voltage-clamp mode. Furthermore, the technique of voltage-clamp-controlled current-clamp is applied for modifying the conventional patch-clamp amplifier, the variable low-pass filter is added into the circuit to reduce the response speed of voltage-clamp module, thus the transient potentials changes can be measured while membrane potential is kept at a constant value. Bridge balance circuitry is designed to eliminate the voltage drop while the variable current injected into the electrode. And fast capacitance compensation stage of conventional PCA is modified to neutralize the capacitance and accelerate system response speed for current-clamp mode. The experimental results on cell model demonstrate that modified PCA meets the requirement of monitoring transient potential changes in electrophysiology research." @default.
- W2392695087 created "2016-06-24" @default.
- W2392695087 creator A5052259052 @default.
- W2392695087 date "2008-01-01" @default.
- W2392695087 modified "2023-09-25" @default.
- W2392695087 title "Design of Voltage-clamp-controlled Current-clamp" @default.
- W2392695087 hasPublicationYear "2008" @default.
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