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- W2392697135 abstract "Objective: To establish a rapid,sensitive,and specific real-time fluorescent quantitative polymerase chain reaction(FQ-PCR) method for quantitative detection of human cytomegalovirus(HCMV).Methods: HCMV IE(immediately gene) fragment was chosen to build FQ-PCR reaction system by creating upstream and downstream primers and TaqMan probe.Plasmids containing target fragments of HCMV were conducted as the external standards to develop the real-time FQ-PCR.Additional reference strains from the bacteria and virus were used to test the specificity of primers and probes,and fifty clinical specimens were quantitative detected with real-time FQ-PCR.Results: The quantitative range of the assays was 1010-106copy/L,R=0.997,and the correlation was well.The concentration of standard coefficient of variation of Ct values was less than 5%,and the reproducibility was well.And specificity was determined by non-amplificated different human pathogens,including bacteria and virus.Fifty clinical specimens collected from patients were assayed by this method,and the detection rate of HCMV was 86%(43/50).Conclusion: The real time FQ-PCR was demonstrated as a rapid,specific and convenient method,and it is helpful in the study of the pathogenesis and epidemiology of HCMV." @default.
- W2392697135 created "2016-06-24" @default.
- W2392697135 creator A5042405892 @default.
- W2392697135 date "2012-01-01" @default.
- W2392697135 modified "2023-10-17" @default.
- W2392697135 title "Establishment of Real-Time FQ-PCR for Quantitative Detection of Human Cytomegalovirus and Its Preliminary Clinical Application" @default.
- W2392697135 hasPublicationYear "2012" @default.
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