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- W2392718897 abstract "Cultured neurons in vitro are important materials for studying the molecular mechanism of neurotoxicity and screening neurotoxicants.Neurons from rats and mice are the most widely used in neurotoxicology.Considering more and more application of Xenopus leavis,an animal model for developmental biology,in toxicology study,we established an in vitro method for culturing brain neurons from X.laevis tadpoles.Brains of X.laevis tadpoles at stage 52~53 were dissected and isolated in the L-15 medium by beating.Scattered cells were plated in poly-lysine-coated 24-well plate and cultured at 22℃.The neurons grew well and primary neuronal network was formed after 72h.Cultured neurons were stained by β2 tubulin 7B9 using immunofluorescence to identify neurite growth and neural network.Compared with the method for culturing rat/mouse brain neurons,this simple and rapid method for X.laevis brain neurons has some advantages,such as a separating process without trypsin,a culturing process without glia cells,and obtaining abundant neurons from several brains at the same time.Therefore,cultured brain neurons from X.laevis tadpoles could be used as an alternate material for rat/mouse brain neurons in studying neurotoxicological mechanisms and screening neurotoxicants." @default.
- W2392718897 created "2016-06-24" @default.
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- W2392718897 date "2010-01-01" @default.
- W2392718897 modified "2023-09-23" @default.
- W2392718897 title "Establishment of an in Vitro Method for Culturing Brain Neurons from Xenopus laevis Tadpoles" @default.
- W2392718897 hasPublicationYear "2010" @default.
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